基于转录组测序分析金银花对干旱胁迫的响应机制OA

Taking Lonicera japonica'Zhengyinhua No.3'as the experimental material,changes in physiological indicators of Lonicera japonica leaves under drought stress through drought treatment were investigated,and key differentially expressed genes using transcriptome sequencing were explored,in order to provide references for further exploration of candidate genes and drought-resistant breeding in Lonicera japonica.The results showed that with increasing drought stress,chlorophyll content decreased continuously;malondialdehyde(MDA),peroxidase(POD),superoxide dismutase(SOD),and soluble protein contents first increased then decreased,while catalase(CAT),proline,and soluble sugar contents continuously increased.Transcriptome analysis revealed 5 824 differentially expressed genes,including 3 011 upregulated and 2 813 downregulated genes.GO database enrichment analysis indicated significant functional clustering in transcription factor regulation and protein transferase activities.KEGG database analysis revealed significant enrichment in amino acid biosynthesis,phenylpropanoid biosynthesis,and photosynthesis pathways.Transcription factor family analysis showed enrichment in BH LH,ERF,and NAC families,with multiple differentially expressed transcription factors(e.g.,LjIF213,LjE133,LjPME2)participating in drought resistance.PCR validation of six candidate genes confirmed expression trends consistent with transcriptome results.