LiCaM在光强调控'西伯利亚'百合罗勒烯合成中的作用OA

Taking Lilium'Siberia'as the experimental material,volatile compounds were collected using the dynamic headspace bagging method,and the floral scent components and their release amounts were quantitatively analyzed by automatic thermal desorption-gas chromatography/mass spectrometry(ATD-GC/MS).The LiCaM gene was cloned,and its expression was detected by qRT-PCR.Combined with the application of exogenous CaCl2,the calcium channel inhibitor LaCl3,and the calmodulin inhibitor W7,the role of LiCaM in light intensity-induced ocimene biosynthesis was investigated,in order to provide references for the targeted regulation of floral scent quality in lily.The results showed that the cloned LiCaM gene had a full length of 450 bp,encoding 149 amino acids,and subcellular localization indicated that it was localized in both the cytoplasm and the nucleus.As light intensity increased,both the expression level of LiCaM and the emission of ocimene showed a significant upward trend.Exogenous CaCl2 treatment significantly promoted LiCaM expression and simultaneously increased ocimene emission,whereas LaCl3 and W7 treatments significantly suppressed LiCaM expression and ocimene emission.These findings indicate that the Ca2+-LiCaM signaling pathway is involved in the regulation of light intensity-induced ocimene biosynthesis in Lilium'Siberia'.