铜掺杂生物活性玻璃双网络水凝胶的制备及性能OA

Objective To explore the preparation method and performance characteristics of a new double-network hydrogel based on carboxymethyl chitosan(CMCS)/silk fibroin(SF)/copper-doped bioactive glass(CuBG).Meth-ods Polyethylene glycol diphenyldehyde(PEGDE)was used as a cross-linking agent to cross-link CMCS,which was then blended with enzymatically cross-linked SF and CuBG nanoparticles to prepare a double-network hydro-gel.The optimal ratio of CMCS/SF/CuBG double-network hydrogels was determined by gelation time and rheologi-cal analysis.The injectability of each group of gel was evaluated through injectability testing and rheological viscos-ity analysis.The swelling properties of the hydrogels were measured using the gravimetric method.The cytocompat-ibility of the hydrogels was evaluated using cell counting kit-8(CCK-8)assay and live/dead cell staining.Results The gelation time of the CMCS single-network hydrogel exceeded 4 minutes,and its mechanical strength was poor.With the addition of SF and CuBG,the gelation time of the hydrogel was shortened,the mechanical strength was enhanced,and the swelling index decreased.Hydrogel injection could form clear and continuous patterns.The CCK-8 and cell live/dead staining results showed that the survival rate of HUVECs cultured in leaching solution of each group of hydrogels was greater than 90%,with complete cell morphology and good proliferation status.Con-clusion The CMCS/SF/CuBG double-network hydrogel exhibits a suitable gelation time,stable mechanical prop-erties,excellent swelling rate,and exhibits no cytotoxicity.These findings indicate that the hydrogel has consider-able potential for biomedical applications.