机械压力对不同部位硬脑膜细胞迁移及促成骨能力的影响OA

Objective To explore the effects of mechanical pressure regulation on the migration and bone-promoting ability of suture dural cells(S-DCs)and non-suture dural cells(NS-DCs).Methods Dural cells(S-DCs),non-cranial suture dural cells(NS-DCs)and cranial suture mesenchymal stem cells(CS-MSCs)of 5-day-old SD rats were isolated and cultured.Four experimental conditions,namely S-DCs-Normal,S-DCs-Stress,NS-DCs-Normal and NS-DCs-Stress,were set according to the source of the cells and whether mechanical pressure(1.07 kPa,24 hours)was applied.The migration ability of dural cells was detected by cell scratch test.Four ex-perimental condition culture media were collected and co-cultured with CS-MSCs for osteogenic induction.Calcium salt deposition was detected by alizarin red staining,and the expression of osteogenesis-related proteins was de-tected by Western blot.Results Compared with the Normal group,the migration abilities of both S-DCs and NS-DCs decreased after Stress treatment(P<0.05).Compared with S-DCs,NS-DCs has a weaker migration ability un-der the same processing conditions(P<0.05).The conditioned medium experiment showed that Stress treatment reduced the osteogenic ability of CS-MSCs(P<0.05),and the osteogenic effect of the conditioned medium derived from NS-DCs was weaker than that of S-DCs(P<0.05).The Western blot results were consistent with the trend of alizarin red staining.Both Stress treatment and NS-DCs sources could down-regulate the expression of osteogenesis-related proteins(P<0.05).Conclusion Mechanical stress can significantly inhibit the migration of dural cells and the osteogenic differentiation process of CS-MSCs mediated by it.The mechanical tolerance and osteogenic regulation ability of S-DCs are significantly better than those of NS-DCs.