仿生超疏水牙科修复树脂材料的构建及其性能研究OA

Objective To evaluate the surface properties,mechanical properties,biocompatibility,and ability to inhibit protein and bacterial of the biomimetic superhydrophobic dental restorative resin material(BSDRM).Meth-ods Nano ZrO2 was modified with fluorosilane(FAS)to obtain FAS-ZrO2,which,together with nano SiO2,was incorporated into a resin matrix composed of bisphenol A-glycerolate dimethacrylate(Bis-GMA),triethylene glycol dimethacrylate(TEGDMA),perfluoroalkyl acrylate(FMA),and a photoinitiator system to fabricate BSDRM.The chemical structure and surface morphology of the material were characterized by Fourier transform infrared spectros-copy(FTIR),X-ray photoelectron spectroscopy(XPS),field-emission scanning electron microscopy(FE-SEM)and atomic force microscopy(AFM).Wettability parameters,including water contact angle,sliding angle and sur-face energy,were measured.Mechanical performance was assessed by Vickers hardness,diametral tensile strength and compressive strength tests.Biocompatibility was evaluated using CCK-8 assays and cell staining.The ability to inhibit protein adsorption and bacterial adhesion were examined by salivary mucin adsorption and Strepto-coccus mutans adhesion assays,respectively.Results Fourier transform infrared spectroscopy(FTIR)and X-ray photoelectron spectroscopy(XPS)confirmed the successful grafting of FAS onto the ZrO2 surface.BSDRM exhib-ited a micro/nanoscale hierarchical roughness,with a water contact angle of 152.97°±1.64°,a sliding angle of 7.37°±1.91°,and a surface energy of(1.20±0.26)mJ/m2.Mechanical testing showed that BSDRM had signifi-cantly higher hardness than the hydrophilic resin and the commercial pit-and-fissure sealant,whereas no statisti-cally significant difference in compressive strength was observed between BSDRM and the commercial pit-and-fissure sealant(P>0.05).CCK-8 and cell staining results demonstrated that BSDRM was non-cytotoxic to L929 cells and preserved normal cellular morphology.In the protein adsorption assay,BSDRM showed the lowest mucin adsorption among all groups(P<0.001).In the bacterial adhesion assay,the amount of S.mutans adhering to the BSDRM surface was significantly lower than that in the control groups(P<0.01).Conclusion BSDRM is success-fully developed in this study.The material combines excellent superhydrophobicity,favorable mechanical proper-ties,good biocompatibility,and marked ability to inhibit protein adsorption and bacterial adhesion.By suppress-ing plaque formation,BSDRM may offer a promising new materials-based strategy for caries prevention.