DAZAP2在乳腺癌中的表达及其生物学功能OA

Objective To investigate the expression of DAZ associated protein 2(DAZAP2)in breast cancer and its effect on the biological behavior of tumor cells.Methods The relationship between DAZAP2 expression and pa-tient survival was analyzed using data from The Cancer Genome Atlas(TCGA)database.Immunohistochemistry(IHC)was performed to detect DAZAP2 expression in breast cancer tissues and paratumoral tissues,and its corre-lation with clinicopathological characteristics was evaluated.Kaplan-Meier analysis was used to assess overall sur-vival(OS)and recurrence-free survival(RFS)of patients with different DAZAP2 expression levels.Stable breast cancer cell lines with DAZAP2 knockdown or overexpression were established.The proliferation ability of breast cancer cells was evaluated by CCK-8 and colony formation assays,while migration and invasion abilities were de-tected by wound healing and Transwell assays.Results Bioinformatics analysis and IHC results showed that DAZAP2 expression was significantly lower in breast cancer tissues than in paratumoral tissue(P<0.001).More-over,DAZAP2 protein expression was negatively correlated with histological grade and lymph node metastasis(P<0.05 for both).Kaplan-Meier survival analysis revealed that low DAZAP2 expression was associated with poor prognosis in breast cancer patients(P<0.05).The CCK-8 assay showed that,compared with the sh-Ctrl group,the proliferative activity of breast cancer cells significantly increased in the sh-DAZAP2-1 and sh-DAZAP2-2 groups(F=52.38,P<0.001),whereas compared with the Flag group,cell proliferative activity significantly de-creased in the Flag-DAZAP2 group(F=41.67,P<0.001).The colony formation assay demonstrated that,com-pared with the sh-Ctrl group,the number of colonies significantly increased in the sh-DAZAP2-1 and sh-DAZAP2-2 groups(F=12.74,P<0.01),whereas compared with the Flag group,the number of colonies was significantly re-duced in the Flag-DAZAP2 group(t=9.86,P<0.001).The wound-healing assay showed that,compared with the sh-Ctrl group,the wound closure rates significantly increased in the sh-DAZAP2-1 and sh-DAZAP2-2 groups(F=18.23,P<0.01),whereas compared with the Flag group,the wound closure rate significantly decreased in the Flag-DAZAP2 group(t=10.42,P<0.001).The Transwell assays showed that,compared with the sh-Ctrl group,the numbers of migrated and invaded cells significantly increased in the sh-DAZAP2-1 and sh-DAZAP2-2 groups(F=55.16 and 68.42,respectively;both P<0.001),whereas compared with the Flag group,the numbers of mi-grated and invaded cells significantly decreased in the Flag-DAZAP2 group(t=6.18 and 5.73,respectively;both P<0.01).Conclusion High DAZAP2 expression can delay the malignant progression of breast cancer by sup-pressing the proliferation,migration,and invasion of breast cancer cells.