朱顶红"花孔雀"组培快繁体系建立OA

This study aimed to establish a stable and efficient tissue culture-based rapid propagation system for Hippeastrum rutilum'Peacock Flower',providing technical support for its large-scale commercial propagation.Using bulb explants of H.rutilum'Peacock Flower'as experi-mental materials,we investigated the effects of explant disinfection duration and plant growth regulator combinations on adventitious bud induc-tion and proliferation,followed by rooting culture,acclimatization,and transplantation.The results showed that disinfection with 75%ethanol for 1 min and 10%NaClO for 15 min resulted in a contamination rate of 22.22%and a survival rate of 100%for the bulb explants.The optimal medium for adventitious bud induction was MS+2 mg/L 6-BA+1 mg/L NAA,achieving an induction rate of 86.67%.The most effective pro-liferation medium was MS+2 mg/L TDZ+2 mg/L 6-BA+1 mg/L NAA,with a proliferation coefficient of 3.67.Adventitious buds exhibited a 100%rooting rate and robust root growth on 1/2 MS+0.2 mg/L NAA+0.5 g/L AC.After transplantation into a substrate mixture of peat and perlite at a 1∶1(v/v)ratio,the survival rate of the tissue-cultured plantlets reached 100%.