高粱品种萌发期耐盐性筛选与鉴定OA

[Objective]Based on the response characteristics of different sorghum varieties to salt stress during the germination stage,sorghum varieties with excellent salt tolerance were selected to ensure normal growth of sorghum in saline-alkali soil environments.[Method]Ten sor-ghum varieties were used as experimental materials,with deionized water treatment serving as the control.A 150 mmol/L NaCl solution was used to simulate salt stress.Germination potential,germination rate,germination index,vigor index,root-to-shoot ratio,as well as fresh weight,dry weight,and length of shoots and roots of individual plants were measured.After calculating the relative values of each index,correlation analy-sis,principal component analysis,and cluster analysis were performed.[Result]Except for root dry weight of individual plants,there were highly significant differences(P＜0.01)among treatments for the other 10 indicators.Variance analysis of the salt tolerance coefficient showed that the increase rate of each index was from-74.6%to 211.7%.Correlation analysis revealed that seed germination-related indicators were significantly positively correlated with most shoot growth indicators,and shoot growth indicators were also significantly positively correlated with root growth indicators,reflecting synergistic associations among various growth traits during sorghum germination.Principal component analysis reduced the 11 original indicators to two pricipal component factors,with a cumulative contribution rate of 69.63%,indicating that these two factors effec-tively captured the core information contained in the original indicators.This study identified germination rate and vigor index as core evaluation indicators for assessing salt tolerance during sorghum germination.Based on comprehensive evaluation using the membership function method and cluster analysis,the 10 tested sorghum varieties were classified into four distinct salt-tolerance groups:one highly salt-tolerant type,five salt-tolerant types,three moderately salt-tolerant types,and one salt-sensitive type.Salt tolerance differed significantly among groups.The most salt-tolerant varieties during germination were accessions 6,85,90,39,18,and 45.[Conclusion]These findings provide support for breeding new salt-tolerant sorghum varieties,genetic improvement,and studies on the molecular mechanisms underlying salt tolerance.