包头市3个湖泊水体微生物群落特征及其驱动因子OA

[Objective]This study aimed to explore the relationship between microbial community structure and environmental factors by comparing and analyzing the physicochemical properties and microbial communities of three water bodies.[Method]Baotou Nanhai Lake,the tailings landscape lake,and Xiaobaihe Lake were selected as study sites,where physicochemical parameters were measured and amplicon sequencing was conducted.[Result]The water in all three sampling sites was weakly alkaline.Electrical conductivity(EC)and total dissolved solids(TDS)ranked as follows:tailings landscape lake>Nanhai Lake>Xiaobaihe Lake,whereas 5-day biochemical oxy-gen demand(BOD5)followed the order:tailings landscape Lake>Nanhai Lake>Xiaobaihe Lake.Nanhai Lake exhibited the lowest water transparency and the highest nutrient content.The cationic composition in all three lakes were dominated by Na+,Mg2+,Ca2+,and K+.The dominant anion in the tailings landscape lake was SO42-,whereas the dominant anions in Nanhai Lake and Xiaobaihe Lake were Cl-and HCO3-,respectively.Bacterial diversity and richness generally ranked as Nanhai Lake>tailings landscape lake>Xiaobaihe Lake,while eukaryotic microbial diversity followed the order:Xiaobaihe Lake>Nanhai Lake>tailings landscape lake.Proteobacteria were abundant at all three sites,and their relative abundances differed significantly among lakes(P<0.05).Pearson correlation analysis indicated that microorganisms in the tailings landscape lake were significantly influenced by various envi-ronmental factors.In Xiaobaihe Lake,the abundance of most microorganisms was correlated with metal salts and anion contents;specifically,the abundances of Bacteroidota(bacteria),Chytridiomycota,and Rozellomycota(fungi)were related to NH4+-N and HCO3-concentrations.In Nanhai Lake,microbial communities other than Chytridiomy-cota were correlated with K+.Hydrogenophaga in the tailings landscape lake exhibited strong sulfate tolerance,con-sistent with the high sulfate concentration in the tailings landscape water.[Conclusion]The water bodies of the three lakes are moderately polluted.pH,salinity,and ion concentrations are the main factors driving differences in micro-bial diversity.These findings provide an experimental basis for developing pollution traceability technology based on microbial indicators and for guiding ecological restoration of water bodies.