中国樱桃WRKY基因家族鉴定及其表达分析OA

[Objective]WRKY transcription factors play crucial regulatory roles in fruit development in horti-cultural plants.This study aimed to systematically characterize the genome-wide features of the WRKY transcription factor family in Chinese cherry(Prunus pseudocerasus(Lindl.)G.Don),analyze its expression patterns during fruit development,and screen for key candidate genes involved in regulating fruit development and ripening.[Method]WRKY gene family members were identified from the Chinese cherry genome,and comprehensive bioinformatics analyses were conducted to explore their physicochemical properties and phylogenetic relationships.Expression pat-terns were investigated using transcriptome data from different fruit developmental stages of three Chinese cherry cul-tivars and validated via RT-qPCR.[Result]Fifty-eight WRKY gene family members were identified in the P.pseu-docerasus genome and designated as PpseWRKY1-PpseWRKY58.These genes were unevenly distributed across eight chromosomes and encoded proteins ranging from 117 to 644 amino acids with isoelectric points of 4.94-9.92;all predicted proteins were hydrophilic.Phylogenetic analysis classified the 58 PpseWRKY genes into three groups:Group I(12),Group II(39),and Group III(7).Conserved motif and gene structure analyses revealed that all PpseWRKY genes contained the conserved WRKYGQK motif,with high similarity in gene structure and conserved motifs within the same group.Numerous cis-acting elements related to hormone regulation,light response,and stress response were enriched in the promoters of PpseWRKY genes.Collinearity analysis identified 15 segmental duplica-tion pairs among PpseWRKY genes.Transcriptome and RT-qPCR analyses showed distinct expression patterns of PpseWRKY genes among different cultivars and fruit developmental stages.Most genes,such as PpseWRKY27,PpseWRKY37,PpseWRKY49,and PpseWRKY53,were highly expressed at the green fruit stage and significantly down-regulated during ripening,suggesting roles in early fruit development.In contrast,the expression levels of PpseWRKY5 and PpseWRKY23 gradually increased during fruit development,implying positive regulatory functions in fruit ripening and quality formation.[Conclusion]This study provides the first systematic characterization of the genome-wide features of the WRKY transcription factor family and their expression profiles during fruit development in Chinese cherry,establishing a theoretical foundation for further functional analysis of fruit development and ripening.