益气解毒方下调PTGES2抑制鼻咽癌细胞迁移与侵袭的机制OA

Objective To investigate the effects of Yiqi Jiedu Prescription(YJP)and prostaglandin E synthase 2(PTGES2)on the migration and invasion of nasopharyngeal carcinoma(NPC)5-8F cells,as well as the role of PTGES2 in the effects of YJP.Methods(1)5-8F cells were divided into a control group,a low-concentration YJP group(0.5 mg·mL-1),a high-concentration YJP group(1.0 mg·mL-1),and a cisplatin group(2 µg·mL-1).Wound healing assay and Transwell invasion assay were used to assess cell migration and invasion abilities.The protein expression levels of PTGES2,Vimentin,N-cadherin,and E-cadherin were determined by Western Blot.(2)5-8F cells were transduced with lentiviral vectors for PTGES2 overexpression(oe-PTGES2)and the corresponding negative control(oe-NC),or for PTGES2-targeting shRNA(sh-PTGES2)and its negative control(sh-NC).The transduced cells were treated with or without YJP(1.0 mg·mL-1).For the overexpression experiments,cells were divided into the oe-NC group,oe-NC+YJP group,oe-PTGES2 group,and oe-PTGES2+YJP group.For the knockdown experiments,cells were divided into the sh-NC group,sh-NC+YJP group,sh-PTGES2 group,and sh-PTGES2+YJP group.Wound healing assay and Transwell invasion assay were used to assess cell migration and invasion abilities,and Western Blot was performed to measure the protein expression levels of PTGES2,Vimentin,phosphorylated AKT(p-AKT),N-cadherin,and E-cadherin.Results(1)Compared with the control group,the high-concentration YJP group and the cisplatin group exhibited significantly lower relative migration and invasion rates(P＜0.01),reduced protein expression of PTGES2,Vimentin,and N-cadherin,and increased expression of E-cadherin(P＜0.05,P＜0.01).(2)After lentiviral transduction,compared with the oe-NC group,the oe-PTGES2 group significantly enhanced relative migration and invasion rates(P＜0.01),along with increased protein expression of PTGES2,Vimentin,p-AKT,and N-cadherin(P＜0.05,P＜0.01),and decreased E-cadherin expression(P＜0.05).Compared with the sh-NC group,the sh-PTGES2 group exhibited reduced relative migration and invasion rates(P＜0.01),downregulated protein expression of PTGES2,Vimentin,p-Akt,and N-cadherin(P＜0.01),and upregulated E-cadherin expression(P＜0.01).When combined with YJP treatment,compared with the oe-NC+YJP group,the relative migration and invasion rates of cells in the oe-PTGES2+YJP group were increased(P＜0.01),the protein expression of p-Akt,and N-cadherin were elevated(P＜0.05,P＜0.01),and the expression of E-cadherin was decreased(P＜0.05).In contrast,compared with the sh-NC+YJP group,the sh-PTGES2+YJP group showed reduced relative migration and invasion rates(P＜0.01),decreased protein expression of PTGES2,Vimentin,p-Akt,and N-cadherin(P＜0.01),and increased E-cadherin expression(P＜0.01).Conclusion PTGES2 can promote the migration and invasion of NPC cells;the inhibitory effect of YJP on the migration and invasion of NPC cells may involve suppression of the PTGES2/AKT pathway.