"调神畅志"针法对左旋多巴诱发异动症大鼠纹状体细胞外信号调节激酶/哺乳动物雷帕霉素靶蛋白信号通路调控的影响OA
Influence of"Tiaoshen Changzhi"acupuncture therapy on the regulation of ERK/mTOR pathway in striatum of rats with levodopa-induced dyskinesia
目的:观察"调神畅志"针法对左旋多巴诱发异动症(levodopa-induced dyskinesia,LID)大鼠症状的改善作用,并探讨其对细胞外信号调节激酶(extracellular regulated protein kinases,ERK)/哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)信号通路的作用机制.方法:采用 6– 羟基多巴胺(6-hydroxydopamine,6-OHDA)脑内立体定向注射术制备帕金森病模型,在此基础上采用腹腔注射左旋多巴/苄丝肼的方式制备 LID 模型.设立正常组、假手术组、模型组、西药组、调神畅志组、普通针刺组.调神畅志组选取"百会穴透太阳穴""印堂穴""中脘穴""巨阙穴"、双侧"三阴交穴"、双侧"太冲穴".普通针刺组选取"舞蹈震颤控制区"、双侧"风池穴"、双侧"合谷穴"、双侧"太冲穴"、双侧"阳陵泉穴".采用阿扑吗啡(apomorphine,APO)诱导行为学实验、异常不自主运动量表(abnormal involuntary movement scale,AIM)评分、左前肢功能评定、对侧旋转实验观察各组大鼠造模后与干预结束后行为学情况;免疫组织化学法检测大鼠纹状体内mTOR、ERK 的表达;实时荧光定量聚合酶链式反应法检测大鼠纹状体内 mTOR mRNA、ERK mRNA 的表达.结果:与模型组相比,西药组、普通针刺组和调神畅志组的对侧旋转行为、AIM 评分显著降低(P＜0.01),且西药组与调神畅志组明显低于普通针刺组(P＜0.05);与模型组相比,调神畅志组、普通针刺组和西药组大鼠纹状体内 ERK、mTOR 蛋白和 ERK mRNA、mTOR mRNA 相对表达量明显降低(P＜0.01),且西药组低于调神畅志组(P＜0.05),调神畅志组低于普通针刺组(P＜0.05).结论:"调神畅志"针法能够改善 LID 大鼠的行为学表现,减少异常不自主运动和对侧旋转行为,其作用机制可能与降低LID 大鼠纹状体内 ERK、mTOR 蛋白表达水平,抑制ERK/mTOR 通路有关.
Objective:To observe the effect of"Tiaoshen Changzhi(调神畅志)"acupuncture therapy on improving the symptoms of levodopa-induced transactivity disorder(LID)rats,and explore the mechanism of the ERK/mTOR signaling pathway.Methods:Parkinson's disease(PD)model was established by stereotaxic intracerebral injection of 6-OHDA,and LID model was further induced by intraperitoneal injection of levodopa/benzylserazid.Six groups:normal group,sham operation group,model group,Western medicine group,Tiaoshen Changzhi group and ordinary acupuncture group.Tiaoshen Changzhi group selected the following acupoints"Baihui(GV20)through to Taiyang(EX-HN5)","Yintang(EX-HN3)","Zhongwan(CV12)","Juque(CV14)",bilateral"Three Yin Jiao(SP6)",bilateral"Taichong(LR3)".The ordinary acupuncture group selected the"dance tremor control area",bilateral"Fengchi(GB2)",bilateral"Hegu(LI4)",bilateral"Taichong(LR3)"and bilateral"Yanglingquan(GB34)".Apomorphine(APO)induced behavioral assessment,abnormal involuntary movement scale(AIM)score,left forelimb function evaluation and the contralateral rotation test were used to observe the behavioral changes in each group after modeling and in each group after intervention.The expression of mTOR and ERK in rat striatal tissue was measured by immunohistochemistry.The expression of mTOR mRNA and ERK mRNA in rat striatal tissue was quantified by qRT-PCR.Results:The contralateral rotation behavior and AIM score of the Western medicine group,the ordinary acupuncture group and Tiaoshen Changzhi group were significantly decreased compared with the model group(P<0.01),and the Western medicine group and Tiaoshen Changzhi group were significantly lower than those in the ordinary acupuncture group(P<0.05).Compared with model group,the relative expressions of ERK,mTOR proteins and ERK mRNA,mTOR mRNA in striatal tissue of rats in Tiaoshen Changzhi group,the ordinary acupuncture group and Western medicine group were significantly decreased(P<0.01),and the Western medicine group was lower than the Tiaoshen Changzhi group(P<0.05),while the Tiaoshen Changzhi group was lower than the ordinary acupuncture group(P<0.05).Conclusion:"Tiaoshen Changzhi"acupuncture can improve the behavioral performance of LID rats,reduce abnormal involuntary movement and contralateral rotation behavior,and its mechanism may be related to reducing the expression levels of ERK and mTOR proteins in the striatum of LID rats and inhibiting the ERK/mTOR signaling pathway.
秦正巍;梁志明
广州医科大学附属中医医院,广东 广州,510000广州医科大学附属第五医院,广东 广州,510000
医药卫生
左旋多巴诱发异动症"调神畅志"针刺纹状体细胞外信号调节激酶/哺乳动物雷帕霉素靶蛋白通路
Levodopa-induced dyskinesia"Tiaoshen Changzhi"acupunctureStriatumERK/mTOR signaling pathway
《中医临床研究》 2026 (20)
1-8,8
广州市科技计划项目(2023A03J0775)广东省针灸学会科研课题(GDZJ2024010)广州市卫健委项目(20241A010085).
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