开郁种玉汤治疗"嫉妒不孕"的网络药理学研究OA
Effects of Kaiyu Zhongyu decoction in the treatment of jealousy-induced female infertility based on network pharmacology
目的:开郁种玉汤是《傅青主女科》"嫉妒不孕"专篇之经典名方,本研究运用网络药理学研究方法探讨开郁种玉汤防治女性心理应激不孕的作用网络与潜在机制.方法:以《中华人民共和国药典》为标准进行药名规范,对组方中中药的性味归经进行分析,筛选开郁种玉汤的主要有效成分与靶点,检索心理应激不孕的相关靶点,构建开郁种玉汤-心理应激不孕的"药物-成分-靶点"作用网络,进行蛋白质-蛋白质相互作用分析,对靶点基因进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析,并进行分子对接.结果:筛选出开郁种玉汤34 个主要有效成分和 947 个预测靶点,心理应激不孕靶点 1 481 个,共同靶点 117 个,如丝氨酸/苏氨酸蛋白激酶 1(Akt serine/threonine kinase 1,AKT1)、白细胞介素(interleukin,IL)-6、肿瘤坏死因子(tumor necrosis factor,TNF)、肿瘤蛋白 p53(tumor protein p53,TP53)、IL-1B 等.通过GO 和KEGG 富集分析得知,这些靶点涉及晚期糖基化终末产物(advanced glycation end product,AGE)-晚期糖基化终末产物受体(advanced glycation end product receptor,RAGE)信号通路、磷脂酰肌醇 3 激酶(phosphoinositide 3-kinase,PI3K)-蛋白激酶 B 信号通路、丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路、IL-17 信号通路等,且 AKT1、IL-6、TNF等核心靶点与药物靶点蛋白对接良好.结论:开郁种玉汤防治心理应激不孕具有多组分、多靶点、多通路的复合机制,通过多种有效成分影响女性下丘脑-垂体-卵巢轴的内分泌和细胞氧化应激反应,从而防治心理应激不孕.
Objective:Kaiyu Zhongyu decoction(开郁种玉汤)is a renowned classical prescription documented in the special chapter on"jealousy-induced female infertility"of FU Qingzhu Nvke(《傅青主女科》).This study aims to explore the network and potential mechanism of Kaiyu Zhongyu decoction in the treatment of"jealousy-induced female infertility"by network pharmacology.Methods:The Pharmacopoeia of the People's Republic of China(《中华人民共和国药典》)was used as thestandard for drug name standardization,and the natures,flavors and meridian tropisms of the Chinese herbal medicines in the formula were analyzed.The main active ingredients and targets of Kaiyu Zhongyu decoction were screened,and the relevant targets of jealousy-induced female infertility were retrieved.The drug-ingredient-target interaction network and protein-protein interaction network of Kaiyu Zhongyu decoction against jealousy-induced female infertility were constructed.Gene Ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed on target genes,and molecular docking was conducted subsequently.Results:A total of 34 main active ingredients and 947 predicted targets of Kaiyu Zhongyu decoction were screened out,along with 1 481 targets related to jealousy-induced female infertility,among which 117 common targets were obtained,including serine/threonine kinase 1(AKT1),interleukin-6(IL-6),tumor necrosis factor(TNF),tumor protein p53(TP53)and IL-1B.GO and KEGG enrichment analyses revealed that these targets were involved in multiple signaling pathways,including the advanced glycation end product(AGE)-receptor for advanced glycation end product(RAGE)pathway,phosphoinositide 3-kinase(PI3K)-protein kinase B(Akt)pathway,mitogen-activated protein kinase(MAPK)pathway and IL-17 signaling pathway.Moreover,core targets such as AKT1,IL-6 and TNF exhibited favorable docking activity with corresponding active ingredients.Conclusion:Kaiyu Zhongyu decoction exerts preventive and therapeutic effects on jealousy-induced female infertility via a multi-component,multi-target and multi-pathway integrated mechanism.Its various active ingredients regulate endocrine functions and cellular oxidative stress responses of the hypothalamic-pituitary-ovarian axis in females,thereby intervening against psychological jealousy-induced female infertility.
李永谦;郭颖
山东中医药大学第一临床医学院,山东 济南,250014山东中医药大学附属医院中西医结合生殖与遗传中心,山东 济南,250011
医药卫生
网络药理学开郁种玉汤心理应激不孕症
Network pharmacologyKaiyu Zhongyu decoctionPsychological stressFemale infertility
《中医临床研究》 2026 (19)
58-66,9
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