基于ICP-MS的不同产地地龙无机元素差异分析及健康风险评估OA

Objective:To study the variability of elemental distribution by determining the contents of 25 inorganic elements in Pheretima from different origins,and to assess the health risk of heavy metals and harmful elements.Methods:Inductively coupled plas-ma mass spectrometry(ICP-MS)was used to determine the contents in 36 batches of collected Pheretima samples,the differences of el-ements were analyzed by chemometrics,the maximum daily intake of heavy metals(EDI),target hazard coefficient(THQ),and carcino-genic risk(CR)were calculated,a health risk assessment for them was conducted.Results:25 inorganic elements had good linear rela-tionships within their respective ranges,and the established method was stable and reliable.The contents of Mg,Al,Fe and Zn in Pheretima were relatively high.Cluster heat map and OPLS-DA analysis showed that samples of the same variety of Pheretima exhibited certain aggregation.Elements such Pb,Cd,As,Ba,Li,Sr,Ni,Zn and Hg might be the key mass differences.EDI,THQ and CR evalua-tions suggested that the heavy metals and harmful elements contained in Pheretima might pose certain health risks when accumulated in the human body over a long period of time,and more attention should be paid.Conclusion:Pheretima is rich in inorganic elements.The content determination method and risk assessment model established in this study can provide references for the quality control and medication safety of Pheretima medicinal materials.