基于叶绿体基因组SSR的栽培箭叶淫羊藿遗传多样性研究OA

Objective:To study the genetic diversity and population structure of Epimedium sagittatum in the major cultivation area of Guizhou province using chloroplast genome-derived simple sequence repeat(cpSSR)markers,so as to provide a molecular basis for germplasm identification and the screening of superior germplasm sources.Methods:The cpSSR primers were developed based on the complete chloroplast genome of Epimedium sagittatum.70 samples from 7 cultivated populations in Guizhou were genotyped using se-lected polymorphic primers.Genetic diversity parameters were calculated with PopGene 32 software.Population genetic structure and clustering analysis were performed using STRUCTURE(v2.3.4)and NTSYS-pc(version 2.10e)software,respectively.Results:The 12 pairs of polymorphis primers amplified a total of 82 alleles,with a high percentage of polymorphism(PPB,91.46%).There were signifi-cant differences in genetic diversity among groups.The Pingrui Village,Congjiang County(PY)population exhibited the highest genetic diversity(Nei's H=0.2017),while the Ceheng County(CH)population showed the lowest(Nei's H=0.0795).UPGMA clustering clearly divided the 70 accessions into two distinct genetic groups,with a high level of genetic differentiation between them.The cluste-ring pattern suggested that genetic differentiation was primarily driven by cultivation practice(under-forest vs field farming)and anthro-pogenic introduction history,rather than geographical distance.Primers SSR 71,EK 385 and others exhibited the highest polymorphism and strong discriminatory power.Conclusion:This study develops and verifies the SSR markers of the chloroplast genome of Epimedium sagittatum,revealing that the cultivated germplasm in Guizhou has rich genetic variations and obvious population differentiation.The core cpSSR primers screened out can be used for the germplasm identification of this species and the construction of core germplasm banks,providing a reference for the resource conservation and variety breeding of Epimedium plants.