锐尖山香圆离体快繁技术研究OA
Study on In Vitro Rapid Propagation Technology of Turpinia arguta
目的:建立锐尖山香圆的离体快繁技术体系,为组培苗规模化繁育提供技术支撑.方法:以锐尖山香圆带腋芽嫩枝为外植体,筛选出最佳的腋芽启动培养基、不定芽诱导和增殖培养基及生根培养基,统计腋芽诱导率、不定芽诱导率、增殖系数、生根率和移栽成活率.结果:锐尖山香圆的最佳腋芽启动培养基为 MS+1.0 mg/L 6-BA+0.05 mg/L NAA,腋芽诱导率达 88.33%;最佳不定芽诱导和增殖培养基为 MS+0.2 mg/L 6-BA+0.05 mg/L GA3+0.03 mg/L NAA,增殖系数达 3.87;最佳生根培养基为 1/2MS+2.0 mg/L IAA+0.5 mg/L NAA+0.2 g/L AC,生根率达 92.67%,移栽后成活率可达 98.56%.结论:成功建立了锐尖山香圆的离体快繁技术体系.
Objective:To establish an in vitro rapid propagation technology system for Turpinia arguta,so as to provide technical support for the large-scale propagation of tissue culture seedlings.Methods:Using the tender branches of the axillary buds of Turpinia arguta as explants,the best axillary bud initiation medium,advanced-bud induction and proliferation medium and rooting medium were screened out.The axillary bud induction rate,advanced-bud induction rate,proliferation coefficient,rooting rate and transplantation sur-vival rate were statistically analyzed.Results:The optimal axillary bud initiation medium for Turpinia arguta was MS+1.0 mg/L 6-BA+0.05 mg/L NAA,and the axillary bud induction rate reached 88.33%.The optimal medium for adventitious bud induction and prolifer-ation was MS+0.2 mg/L 6-BA+0.05 mg/L GA3+0.03 mg/L NAA,with a proliferation coefficient of 3.87.The optimal rooting medium was 1/2MS+2.0 mg/L IAA+0.5 mg/L NAA+0.2 g/L AC,with a rooting rate of 92.67%and a survival rate of 98.56%after trans-plantation.Conclusion:The in vitro rapid propagation technology system of Turpinia arguta has been successfully established.
戴小英;张云童;徐小强;程强强;陶秀花;刘新亮
江西省林业科学院,江西 南昌 330032江西省林业科学院,江西 南昌 330032||北京林业大学生物科学与技术学院,北京 100083江西省林业科学院,江西 南昌 330032江西省林业科学院,江西 南昌 330032江西省林业科学院,江西 南昌 330032江西省林业科学院,江西 南昌 330032
医药卫生
锐尖山香圆离体快繁不定芽增殖生根
Turpinia arguta(Lindl.)Seem.In vitro rapid propagationAdventitious budProliferationRooting
《中药材》 2026 (3)
577-581,5
江西省重大科技研发专项项目(20233AAE02017)江西省林业局科技创新重点攻关项目(创新专项[2023]1号)江西省林科院基础研究与人才科研专项(2023522001)
评论