修根对乌头根系细菌群落组成和生物碱积累的影响OA

Objective:To study the microecological mechanism by which root pruning promotes the composition of bacterial commu-nities in Aconitum carmichaelii root system and the accumulation of alkaloids in Aconitum carmichaelii sub roots,so as to provide a sci-entific basis for revealing the impact of root pruning on the quality formation of Aconiti Lateralis Radix Praeparata.Methods:In the field experiments,different root pruning times were set up for treatment.The alkaloid content of Aconitum carmichaelii sub roots was deter-mined by ultraviolet spectrophotometry and high performance liquid chromatography.The bacterial community of Aconitum carmichaelii root system was analyzed by 16S rDNA amplification sequencing.LEfSe combined with correlation analysis screened out biomarkers that significantly positively correlated the microbial classification levels of Aconitum carmichaelii root system treated with different root prun-ing times with alkaloid accumulation.Field trials inoculated key biomarkers to verify their significant role in the accumulation of alka-loids in Aconitum carmichaelii sub roots.Results:Root pruning treatment significantly increased the contents of total alkaloids,benzoyl neoaconitum alkaloid,benzoyl aconitum alkaloid in Aconitum carmichaelii sub roots.The total alkaloid content of Aconitum carmichaelii sub roots pruned twice increased by 64.47%to 68.92%,and root pruning also affected the diversity and composition of Aconitum car-michaelii root system bacteria.Twenty-five biomarkers were positively correlated with the content of two or more alkaloid monomers,and twenty biomarkers were significantly positively correlated with the total alkaloid content.Biomarkers mainly belong to the Firmicutes,Proteobacteria,Actinobacteria,etc.Multiple taxonomic units(Bacillales,Bacillaceae and Bacillus)under Firmicutes were significantly positively correlated with the total alkaloid or specific alkaloid monomer content of Aconitum carmichaelii sub roots,serving as key bio-markers.Field indirect bacteria experiments verified that Bacillus subtilis JY-7-2L could significantly increase the total alkaloid content of Aconitum carmichaelii sub roots by 9.34%to 14.20%.Conclusion:Root pruning affects the composition of the bacterial community in the root system of Aconitum carmichaelii,enriches the bacterial colonies involved in the accumulation of alkaloids of Aconitum car-michaelii sub roots,and thereby ensures and enhances the quality of the Aconiti Lateraitis Radix Praeparata.