微生物菌剂对肉桂生长、有效成分积累及根际细菌群落结构的影响OA

Objective:To explore the effects of microbial inoculants on the growth,active component and rhizobacterial community of Cinnamomum cassia.Methods:Pot experiment was conducted using two-year-old Cinnamomum cassia seedlings.Four treatments were established:sterile water irrigation control(CK),single inoculation with Azospirillum brasilense(A),single inoculation with Bacillus megaterium(B),dual inoculation(AB).Results:Microbial inoculants significantly promoted plant growth.Compared to CK,plant height under A,B,and AB treatments increased by 124%,107%,110%,respectively(P<0.05).The chlorophyll contents of leaves treated A and B were also significantly higher than that of CK,with increases of 75.82%and 72.53%respectively(P<0.05).Inoculant treatment significantly promoted the accumulation of active components in Cinnamomum cassia.Among them,treatment A significantly increased the contents of total flavonoids,total phenols,cinnamaldehyde,cinnamyl alcohol and cinnamic acid,with increases of 9.38%,5.36%,5.14%,33.13%,6.80%,respectively(P<0.05).Microbial inoculants treatment reduced the Alpha diversity index of the rhizosphere soil bacterial community.However,it significantly increased the relative abundance of genera with potential growth-promoting functions(such as the Burkholderia-Caballeronia-Paraburkholderia complex,Rhodanobacter and Chujaibacter,etc).Conclusion:Azospirillum brasilense shows the best comprehensive effect in promoting the growth of Cinnamomum cassia,improving its quality and regulating the beneficial flora in the rhizosphere,and has the potential for development as a highly efficient biological agent specifically for Cinnamo-mum cassia.