钙镁硫配施对北苍术生长、药效物质积累及根际土壤微生态的影响OA

Objective:To explore the regulatory effects and mechanisms of the rational application of medium element(calcium Ca,magnesium Mg,sulfur S)fertilizers on the quality formation of Atractylodis Rhizoma from the perspectives of growth and development,biosynthesis of active substances,and soil microbial diversity.Methods:This study adopted a field experiment,taking biennial Atrac-tylodes chinensis as the research object.Nine fertilization treatment groups with Ca,Mg and S fertilizers were set up.In the later stage of plant growth(the early stage of yellowing and wisteria),the photosynthetic intensity of Atractylodes chinensis was measured using a port-able photosynthesis meter.The contents of atractylodin,atractylon,β-eucalyptol and alatractylodes Ⅰ in the rhizome of Atractylodes chinensis were determined by high performance liquid chromatography.The diversity of bacteria in the rhizosphere soil of Atractylodes chinensis was analyzed based on 16S rDNA high-throughput sequencing technology.Results:Compared with the CK group,the combined application of Ca,Mg and S fertilizers had no significant promoting effect on the plant height and stem thickness of Atractylodes chinensis(P>0.05),but the fresh and dry weights of the roots and stems of Atractylodes chinensis in the T3 group increased significantly by 74.25%and 72.47%respectively(P<0.05).Group T3 significantly increased the net photosynthetic rate(Pn)and transpiration rate(Tr)of Atractylodes chinensis,which could alleviate its"photosynthetic noon break"effect to a certain extent.The combined application of calcium,magnesium and sulfur at different concentrations was significantly beneficial to the accumulation of active substances of At-ractylodes chinensis.Moreover,in group T3,the contents of atractylodin,atractylon,β-eucalyptol and alatractylodesⅠ were significantly increased by 268.00%,368.98%,236.07%and 83.96%respectively(P<0.05).Compared with the CK group,the relative abundance of dominant phyla such as Proteobacteria,Acidobacteriota,Planctomycetota and Patescibacteria in the T3 group was significantly in-creased.The relative abundance of Bacteroidota,Verrucomicrobiota and others was significantly decreased(P<0.05).The relative abun-dance of beneficial bacterial communities such as Burkholderia,Sphingomonas,Bradyrhizobium in T3 group was significantly increased(P<0.05).Diversity analysis revealed that T3 group could improve the richness and diversity of soil bacteria by increasing the abun-dance of beneficial bacteria.Conclusion:T3 group(Ca 4.53 kg/hm2,Mg 5.06 kg/hm2,S 6.67 kg/hm2)can effectively increase the ac-cumulation of dry matter in the roots and stems of Atractylodes chinensis and the synthesis of medicinal substances by improving soil mi-croecology,promoting photosynthesis of Atractylodes chinensis,and alleviating the photosynthetic midday rest of plants.