极早熟玉米自交系CAVA119穗部相关性状遗传解析OA
Genetic Analysis of Ear-related Traits in the Very Early-maturing Maize Inbred Line CAVA119
极早熟玉米区是我国重要的商品粮生产基地之一.近年来,通过增加种植密度来提高极早熟玉米产量的方式日益受限,如何提升单株产量成为突破高产瓶颈的重要途径.然而,当前针对极早熟玉米穗部相关性状遗传位点的研究仍开展较少.为此,以极早熟自交系CAVA119与中早熟自交系2104113005杂交,通过单倍体技术构建双单倍体(DH,doubled haploid)群体,利用Maize 3K液相芯片进行基因型检测,在3个不同环境下对DH系及与其测验种R55921构建的杂交种群体进行穗长、穗粗、穗行数和行粒数等穗部相关性状的QTL定位分析,共检测到86个与穗部性状相关的QTL.其中,在DH群体中,共检测到55个QTL,与穗长、穗粗、穗行数和行粒数相关的QTL分别有17个、16个、15个和7个;效应最大的位点为qKNPR1-2,其LOD值为22.02,表型贡献率为12.02％.在杂交种群体中,共检测到31个穗部相关QTL,与穗长、穗粗、穗行数和行粒数相关的QTL分别有8个、14个、5个和4个.杂交种群体QTL定位结果中有6个QTL与DH群体定位结果重合.此外,还鉴定出2个在多环境下稳定表达的新位点,qEL7-1与qKNPR7-1.本研究为深入解析极早熟玉米穗部相关性状的遗传基础及分子育种提供了重要的理论基础和基因资源.
The extremely early-maturing maize production region is one of the major commercial grain production bases in China.In recent years,increasing planting density to improve the yield of extremely early-maturing maize has become increasingly difficult,making the improvement of yield per plant an important strategy for overcoming the bottleneck of high yield.However,studies on the genetic loci associated with ear-related traits in extremely early-maturing maize remain limited.In this study,a doubled haploid(DH)population was developed through haploid technology using a cross between the extremely early-maturing inbred line CAVA119 and the medium-early-maturing inbred line 2104113005.Genotyping was performed using the Maize 3K liquid SNP chip.QTL mapping analyses for ear-related traits,including ear length,ear diameter,ear row number,and kernel number per row,were conducted in the DH population and corresponding testcross populations R55921 across three different environments.A total of 86 QTLs associated with ear-related traits were identified.In the DH population,55 QTLs were detected,including 17,16,15,and 7 QTLs associated with ear length,ear diameter,kernel row number,and kernel number per row,respectively.The major-effect locus was qKNPR1-2,with a LOD score of 22.02 and a phenotypic variation explained(PVE)of 12.02％.Among these,18 yield-increasing alleles were derived from the extremely early-maturing parent CAVA119.In the testcross population,31 ear-related QTLs were identified,including 8,14,5,and 4 QTLs associated with ear length,ear diameter,kernel row number,and kernel number per row,respectively.Among these,11 yield-increasing alleles were contributed by the extremely early-maturing parent CAVA119.In addition,six QTLs identified in the testcross population overlapped with those detected in the DH population.Furthermore,two novel loci showing stable expression across multiple environments,qEL7-1 and qKNPR7-1,were identified.This study provides important theoretical insights and valuable genetic resources for elucidating the genetic basis of yield-related traits in extremely early-maturing maize and for advancing molecular breeding efforts.
晏宣军;付俊杰;范开建;史海潮;黄玉迪;董雨杭;崔钰;谢玉心;王春超;孙丽芳;黎亮
海南大学热带农林学院(农业农村学院、乡村振兴学院),儋州 571737||中国农业科学院作物科学研究所,北京 100081海南大学热带农林学院(农业农村学院、乡村振兴学院),儋州 571737||中国农业科学院作物科学研究所,北京 100081中国农业科学院作物科学研究所,北京 100081中国农业科学院作物科学研究所,北京 100081||山西农业大学农学院,晋中 030801黑龙江八一农垦大学农学院,大庆 163000黑龙江八一农垦大学农学院,大庆 163000中国农业科学院作物科学研究所,北京 100081中国农业科学院作物科学研究所,北京 100081中国农业科学院作物科学研究所,北京 100081黑龙江八一农垦大学农学院,大庆 163000中国农业科学院作物科学研究所,北京 100081||中国农业科学院国家南繁研究院,海南三亚 572024
极早熟玉米穗部相关性状QTL定位
very early-maturing maizeear-related traitQTL mapping
《植物遗传资源学报》 2026 (9)
1794-1807,14
国家自然科学基金(32272190)中国农业科学院南繁专项(YBXM2531)中国农业科学院创新工程(2060299-2-24)National Natural Science Foundation of China(32272190)Nanfan Special Project,CAAS(YBXM2531)Innovation Program of the Chinese Academy of Agricultural Sciences(2060299-2-24)
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