稻瘟病菌内切几丁质酶基因MoENCH1的功能分析OA

To investigate the function of the endochitinase(ENCH)gene MoENCH1 in rice blast fungus Magnaporthe oryzae,a MoENCH1 knockout mutant was generated using the split-marker approach,and a complemented strain was subsequently constructed.The biological functions of MoENCH1 were systematically characterized,and the molecular mechanisms underlying its role in pathogenicity were further investigated with transcriptome sequencing.The results showed that,compared with the wild-type strain,the MoENCH1 knockout mutant significantly reduced growth rates on oatmeal agar(OMA),complete medium,and minimal medium.Sporulation on OMA was significantly decreased,accompa-nied by reduced conidial germination and appressorium formation rates.In addition,the mutant was insensitive to the cell wall stress agent Congo red and exhibited significantly reduced pathogenicity toward both barley and rice.Transcriptome analysis indicated that MoENCH1 is involved in regulating pathways related to autophagy,peroxisomes,ubiquitin-mediated proteolysis,and glycerophospholipid metabolism in M.oryzae.These results demonstrate that MoENCH1 plays important roles in regulating hyphal growth,sporulation,conidial germination,appressorium formation,and pathogenicity in M.oryzae.