花生蔗糖含量相关基因的定位及KASP标记的开发与验证OA
Mapping of genes-related to sucrose content in peanut and development and validation of KASP markers in peanut
花生籽仁蔗糖含量是评价食用花生的重要品质指标.本研究以高蔗糖花生品种冀花甜 1 号和低蔗糖花生品系JM135 杂交构建的F2 分离群体为材料,利用BSA重测序定位与花生蔗糖含量相关的QTL.结果表明,F2 群体单株籽仁蔗糖含量最大值为 8.54%,最小值为 2.89%,表现为连续变异,符合正态分布.经BSA重测序分析初步定位,鉴定到 6 个候选区间,分别位于Arahy.04 和Arahy.16 染色体上.开发出 2 个KASP标记 16-1475 和 16-1522,可以对花生籽仁蔗糖含量性状进行分子标记辅助选择.根据基因注释信息筛选出 2 个候选基因,表达谱分析表明,Arahy.RUR3FB和 Arahy.LJ1TN7 可能是调控花生蔗糖含量的候选基因.本研究为挖掘花生蔗糖含量相关基因提供了理论支持,为通过分子育种手段选育高糖花生新品种提供了技术支撑.
In this study,an F2 segregation population derived from a cross between the high-sucrose peanut variety"Jihuatian 1"and the low-sucrose peanut line"JM135"was utilized to map QTLs associated with sucrose content via BSA resequencing.The results showed that the kernel sucrose content in the F2 population ranged from 2.89%to 8.54%,exhibiting continuous variation and a normal distribution.Through BSA-seq analysis,six candidate intervals were initially identified,on chromosomes Arahy.04 and Arahy.16.Two KASP markers,16-1475 and 16-1522,were developed for marker-assisted selection(MAS)of the kernel su-crose content in peanut.Based on gene annotation information,two candidate genes were screened.Expression profile indicated that Arahy.RUR3FB and Arahy.LJ1TN7 are potential candidate genes regulating sucrose content in peanuts.This study provides theoretical support for mining genes related to sucrose content in peanuts and offers technical support for breeding new high sugar peanut varieties through molecular breeding approaches.
潘丽娟;迟晓元;姜骁;许静;殷祥贞;万菲菲;孙淑娟;赵旭红;陈娜;马俊卿
山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100滨州市农业技术推广中心,山东 滨州 256601滨州市农业技术推广中心,山东 滨州 256601山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100山东省花生育种重点实验室/山东省花生研究所,山东 青岛 266100
花生蔗糖含量BSA-seq基因定位KASP标记
peanutsucrose contentBSA-seqgene mappingKASP marker
《作物学报》 2026 (9)
2598-2607,10
本研究由山东省重点研发计划项目(2025CXPT167,2025LZGC020),财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-13),山东省农业科学院农业科技创新工程项目(CXGC2026C41,CXGC2025F19,CXGC2025C19),泰山学者工程项目(tstp20240523,tsqn202312292),山东省自然科学基金项目(ZR2023QC177,ZR2023QC146,ZR2025QC282),农业农村部油料作物生物学与遗传育种重点实验室项目(KF2024007)和青岛市自然科学基金青年项目(25-1-1-38-zyyd-jch)资助.This study was supported by the Key Research and Development Project of Shandong Province(2025CXPT167,2025LZGC020),the China Agriculture Research System of MOF and MARA(CARS-13),the Innovation Project of SAAS(CXGC2026C41,CXGC2025F19,CXGC2025C19),the Taishan Scholars Program(tstp20240523,tsqn202312292),the Natural Science Fund of Shandong Province(ZR2023QC177,ZR2023QC146,ZR2025QC282),the Open Project of Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs(KF2024007),and the Qingdao Natural Science Foundation Youth Project(25-1-1-38-zyyd-jch).
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