植物原生质体制备优化及其单细胞电感耦合等离子体质谱应用挑战OA

Single cell-inductively coupled plasma-mass spectrometry(SC-ICP-MS)can accurately analyze the element distribu-tion in plant tissues at the single-cell level.This technology can explore the cell heterogeneity ignored by traditional population analysis methods,significantly improve the spatial resolution of elemental analysis,and provide a new way for elemental quanti-fication.However,SC-ICP-MS determination technology is limited by the acquisition of high-quality cell suspension and accurate elemental analysis.Current studies have shown that the preparation of high-quality protoplasts is a critical prerequisite for the application of this technology.Therefore,the establishment of an efficient and stable plant protoplast preparation method directly determines the sensitivity,quantitative accuracy and repeatability of SC-ICP-MS detection,which has become a prerequisite for restricting the application of this technology.In this review,the preparation process of common plant protoplasts and their core adaptation principles to SC-ICP-MS were introduced,including source tissue selection,pretreatment,optimization of enzymatic hydrolysis system(ratio of enzymatic hydrolysate,digestion time,osmotic pressure regulation and addition of auxiliary sub-stances),protoplast purification,vitality detection,and chemical fixation.The cell-level research on nutrients and heavy metals was summarized as well as the parameter optimization strategies of SC-ICP-MS were summarized.This technique is still faced with challenges in a proper balance between cell integrity and element retention,besides the possibility of accounting for the to-xicity effects,uptake,and biological effects of exogenous substances.Future research should combine imaging,omics and other technologies to explore the metabolic process of elements,and further promote the application of SC-ICP-MS detection in agri-culture,environment,ecology and life sciences.