基于网络药理学、分子对接及实验验证探讨清咽利喉方治疗急性咽炎的作用机制OA

Objective:To explore the mechanism of Qingyan Lihou Formula in treating acute pharyngitis based on network pharmacology and molecular docking,and to validate the findings through animal experiments.Methods:Active ingredients and corresponding targets of Qingyan Lihou Formula were screened using data-bases such as TCMSP and Herb.Targets related to acute pharyngitis were obtained from the GeneCards and Drugbank databases.After intersecting the targets,a"Chinese herbal medicine-active ingredient-target"network and a protein-protein interaction(PPI)network were constructed.Gene Ontology(GO)functional annotation and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed,and mo-lecular docking was used to predict the binding affinity between key components and core targets.In the animal experiment,a rat model of acute pharyngitis was established by 15%ammonia spray.Rats were ran-domly divided into normal control group,model control group,Pudilan oral liquid group,and high-does and low-does groups of Qinyan Lihou Formula.After 6 days of administration,the pharyngeal macroscopic sta-tus and histopathological changes were observed.The expression levels of PI3K,p-PI3K,AKT,and p-AKT in pharyngeal tissues were detected using the Wes system.Results:Network pharmacology analysis identified 62 active ingredients from Qingyan Lihou Formula and 208 corresponding targets associated with acute phar-yngitis.The core targets included AKT1,PI3K,and IL-6.KEGG enrichment analysis indicated that the PI3K-AKT signaling pathway was the key pathway.Molecular docking results showed that active ingredients such as quercetin and methylophiopogonone A exhibited good binding affinity with PI3K and AKT proteins,with binding energies all less than-6.0 kcal/mol.Animal experiments demonstrated that compared with the model control group,all Qingyan Lihou Formula dose groups showed significantly improved pharyngeal secre-tions,congestion,and swelling,as well as reduced histopathological damage in pharyngeal tissues.The expres-sion levels of PI3K,p-PI3K,AKT,and p-AKT in pharyngeal tissues were significantly decreased(P＜0.05,P＜0.01).Conclusion:Qingyan Lihou Formula may exert its therapeutic effects on acute pharyngitis by regu-lating the PI3K-AKT signaling pathway through active ingredients such as quercetin and methylophiopogo-none A to suppress inflammatory responses.