丝瓜种子形态多样性评价及其核糖体失活蛋白含量比较OA

To investigate seed morphological diversity in Luffa and its relationship with ribosome-inactivating protein(RIP)content,this study analyzed eight seed traits—length,width,length-to-width ratio,circumference,area,thickness,hundred-seed weight,and color—of 175 Luffa cylindrica and 49 Luffa acutangula germplasm collected do-mestically and internationally.Based on seed color and clustering results,the RIP content of seeds from 21 randomly selected Luffa germplasm was subsequently determined.The results showed that most Luffa cylindrica germplasm had black seeds,accounting for 80.57％of the total,while all Luffa acutangula germplasm exhibited black seeds.Among the quantitative traits,seed width and hundred-seed weight showed the highest coefficients of variation in Luf-fa cylindrica,while the length-to-width ratio showed the lowest.For Luffa acutangula,hundred-seed weight and circ-umference had the highest and lowest coefficients of variation,respectively.Correlation analysis revealed extremely significantly(p<0.01)positive correlations among seed length,circumference,area and hundred-seed weight in both Luffa cylindrica and Luffa acutangula.Stepwise regression analysis further indicated that seed area and thick-ness together explained more than 50.0％of the variation in hundred-seed weight in both species.Principal compo-nent analysis and cluster analysis not only clarified the correlations among morphological traits but also classified Luf-fa cylindrica into four groups and Luffa acutangula into three groups.The RIP content in the Luffa acutangula was significantly(p<0.05)higher than that in the Luffa cylindrica,but no significant difference was observed among different groups of the same germplasm.Additionally,darker-colored Luffa seeds contained higher RIP contents than white ones.The results of this study provide a scientific basis for the evaluation,conservation,and innovative utiliza-tion of Luffa germplasm resources.