35 份贵州茶树种质资源的生化成分多样性分析与综合评价OA

To explore the quality characteristics of biochemical components in natural hybrid progenies derived from several local landraces and tea cultivar Huangjinya,and to supply theoretical references for future novel tea cultivar breeding,high-performance liquid chromatography(HPLC)was adopted to quantify 28 biochemical components across 35 tea accessions conserved in the germplasm nursery of Guizhou Tea Research Institute.Multiple statistical approaches including genetic diversity analysis,correlation analysis,principal component analysis(PCA)and clus-ter analysis were integrated to conduct a comprehensive evaluation of all tested accessions.The results revealed that the average coefficient of variation(CV)of the 28 biochemical components reached 43.04％.Cysteine(Cys)con-tent exhibited the maximum CV of 117.40％,while caffeine(CAF)content showed the minimum CV of 14.55％.The mean value of the genetic diversity index was 1.81;phenylalanine(Phe)content possessed the highest index value of 2.09,and gallocatechin gallate(GCG)content had the lowest value of 0.66.Overall,amino acid compo-nents presented relatively high genetic diversity indexes.Seven principal components were extracted via PCA,ac-counting for a cumulative variance contribution rate of 78.451％.Total catechins(TC),arginine(Arg),epigallo-catechin gallate(EGCG),glutamic acid(Glu),epicatechin gallate(ECG)and theanine(The)contents were identified as the core evaluation indicators.All tested accessions were divided into 4 groups by cluster analysis.GroupⅣ,characterized by high amino acid contents as well as low alkaloid and catechin levels,obtained the optimal comprehensive score and possessed excellent suitability for manufacturing premium green tea.Based on the integrated evaluation results,9 elite accessions represented by GT-AS-70 and GT-AS-83 were screened for developing green tea-specific cultivars.