基于基因芯片的珍珠糯基因组分析和性状解析OA

The conventional Oryza sativa subsp.indica variety Zhenzhunuo is characterized by stable yield,excellent grain quality,and relatively strong resistance to rice blast and bacterial leaf blight,making it an important variety in the waxy rice industry of the southern Henan rice region.To deeply dissect the genetic basis underlying the formation of agronomic traits in Zhenzhunuo,its genome was systematically analyzed using the GSR 40K SNP high-density rice whole-genome chip.The results showed that the genomic homozygosity of Zhenzhunuo reached 98.21％,and the proportion of Oryza sativa subsp.indica-type fragments was 70.75％,indicating a typical rice variety of Oryza sativa subsp.indica genetic background.Functional gene analysis revealed that Zhenzhunuo carried multiple yield-related genes(CYP704A3,GL3.2,GW8,Gn1a,GNP1),biotic stress resistance genes(Xa3,Xa26,Bph3),plant architecture-related genes(D2,TAC3,TIG1),and nutrient use efficiency genes(NRT1.1B,OsHKT2;1).Haplotype analysis confirmed that Zhenzhunuo simultaneously carried the rice blast resistance genes Pia,Pid2 and Pid.The pyramiding of these favorable genes provided a molecular basis for its high and stable yield,strong stress resistance,compact plant archi-tecture,and efficient nutrient utilization.However,Zhenzhunuo had deficiencies in the presence of the fragrance gene Badh2,broad-spectrum and durable rice blast resistance genes(such as Pigm and Pi9),and the number of major high-yield genes.