防风AP2/ERF转录因子家族鉴定与表达分析OA

To investigate the expression patterns of SdAP2/ERF in 2 provenances of Saposhnikovia di-varicata and to screen AP2/ERF transcription factors influencing the differences in growth character-istics between the different provenances,bioinformatics methods were employed to identify and func-tionally analyze the AP2/ERF gene family in Saposhnikovia divaricata.The expression patterns of these genes in different provenances of Saposhnikovia divaricata were validated by qRT-PCR to study their roles in the growth and development of Saposhnikovia divaricata and to identify key regulatory genes.The results showed that 208 AP2/ERF genes were identified from the Saposhnikovia divarica-ta genome and classified into five subfamilies(AP2,ERF,DREB,RAV,and Soloist).Their encoded proteins contained one or more conserved AP2 domains and were generally unstable and hydrophilic.Motif analysis revealed that,apart from the AP2/ERF domain,other conserved regions were selec-tively distributed among different clades of the phylogenetic tree.Cis-acting element analysis showed that the promoter regions of the AP2/ERF family genes were significantly enriched in regulatory ele-ments associated with light response,plant hormone signal transduction,and abiotic stress responses.Chromosomal localization results indicated that the AP2/ERF genes were unevenly distributed across eight chromosomes.Intraspecific collinearity analysis revealed 71 collinear gene pairs among the AP2/ERF genes.Interspecific collinearity analysis showed that the SdAP2/ERF genes were evolutionarily closer to the homologous genes of Daucus carota L.Transcriptome analysis demonstrated that,com-pared with BC provenance of Saposhnikovia divaricata,significantly differentially expressed genes were observed in the CC provenance,with SdERF08,SdERF07,SdERF09 and SdERF28 showing significantly high expression,which could be closely associated with the growth characteristics of Sa-poshnikovia diuaricata.