基于转录组测序的金银花抗白粉病分子机制研究OA
Research on Molecular Mechanism of Lonicera japonica Resistance to Powdery Mildew based on Transcriptome Sequencing
为探究金银花应对白粉病的分子机制,以金银花新品系郑银花6号为试材,采用高通量转录组测序方法,研究白粉病侵染金银花叶片差异表达基因变化和代谢通路情况.结果表明,差异表达基因1 225个,其中上调基因1 124个,下调基因101个;KEGG分析,结果表明,显著富集上调的代谢通路分别为植物激素信号传导、植物病原菌相互作用、苯丙烷代谢、角质层、木栓层和蜡质的生物合成等通路.对6个候选基因乙烯受体(Lj2P1103T21)、油菜素内酯-酰胺合成酶(Lj9A591T86)、ATP 酶(Lj2A1239T190)、脂肪酰基-CoA 还原酶(Lj2A985G11)、糖基水解酶家族(Lj6C57T5)和苯丙氨酸氨裂解酶(Lj3A983T60)进行PCR验证,基因表达趋势与PCR验证结果一致.
To explore the molecular mechanism of Lonicera japonica response to powdery mildew,using the new strain Zhengyinhua No.6 as the test material,a high-throughput transcriptome sequen-cing method was employed to study the changes in differentially expressed genes and metabolic path-ways in Lonicera japonica leaves after infection by powdery mildew.The results showed that there were 1 225 differentially expressed genes,among which 1 124 were up-regulated and 101 were down-regulated.KEGG analysis revealed that the significantly enriched up-regulated metabolic pathways in-cluded plant hormone signaling,plant pathogen interaction,phenylpropanoid metabolism,cuticle,periderm layer,and wax biosynthesis,etc.For the 6 candidate genes-ethylene receptor(Lj2P1103T21),brassinolide-amide synthase(Lj9A591T86),ATPase(Lj2A1239T190),fatty ac-yl-CoA reductase(Lj2A985G11),glycosyl hydrolase family(Lj6C57T5),and phenylalanine ammo-nia-lyase(Lj3A983T60)-PCR verification was conducted,and the gene expression trends were con-sistent with the PCR verification results.
张晓申;吴小波;田芳;申潇潇;曹辉;左红娟;申瑞楠
郑州市农业科技研究院,郑州 450005郑州市农业科技研究院,郑州 450005郑州市农业科技研究院,郑州 450005郑州市农业科技研究院,郑州 450005郑州市农业科技研究院,郑州 450005郑州市农业科技研究院,郑州 450005郑州市农业科技研究院,郑州 450005
农业科技
金银花白粉病转录组分析分子机制
Lonicera japonicapowdery mildewtranscriptome analysismolecular mechanism
《种子》 2026 (8)
54-59,6
河南省科技兴林项目"金银花新品种选育及提质增效栽培技术研究与示范"(豫财环资[2025]44号)
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