油莎豆CNR基因家族分析及CeCNR4调控块茎膨大的功能鉴定OA

Cyperus esculentus is an important tuberous oil crop,and its tuber size directly affects yield.The Cell Number Regulator(CNR)gene family plays a key role in regulating plant organ size,but its role in Cyperus esculentus remained poorly understood.To elucidate the potential function of CeCNR4 in tuber expansion,20 members of the CeCNR gene family(CeCNR1-CeCNR20)were identified in Cyperus esculentus using bioinformatics approaches,and their conserved domains,conserved motifs,and phylogenetic relationships were analyzed.The results showed that the CeCNR gene family was highly conserved in evolution.The CeCNR4 and CeCNR15 were highly homologous to the known or-gan size-regulating CNR genes from Solanum lycopersicum L.)and Zea mays L..Furthermore,ex-pression pattern analysis of CeCNRs revealed that the expression level of CeCNR4 was negatively cor-related with tuber tuber expansion,suggesting that CeCNR4 could be a member of the CNR gene family that negatively regulates tuber tuber expansion in Cyperus esculentus.By constructing 35S∷CeCNR4 overexpression transgenic Arabidopsis thaliana(L.)Heynh.plants,it was found that both plant height and silique length were significantly reduced compared with those of the wild type,con-firming that CeCNR4 was a negative regulator that inhibits plant organ growth.