樟树ARF基因家族的全基因组鉴定与表达分析OA

Auxin response factors(ARF),as plant-specific transcription factors,play key roles in per-ceiving auxin signals and regulating various biological processes,including flower development,later-al root formation,stress responses,and secondary metabolite accumulation.However,the functions of the ARF gene family in Cinnamomum camphora,an economically important tree species with sig-nificant medicinal and aromatic value,remained poorly understood.Through bioinformatics approa-ches,24 CcARF genes were identified at the genome-wide level and classified into 5 subgroups,which were unevenly distributed across 12 chromosomes.Analysis of conserved motifs and gene structures revealed high conservation within the same subgroup.Cis-acting element analysis of the promoters in-dicated that these genes were involved in multiple hormone and stress responses.Expression profiling revealed tissue-specific expression patterns,predominantly concentrated in stems.Co-expression anal-ysis showed that several CcARF genes were highly co-expressed with key terpene synthase genes,such as the borneol diphosphate synthase gene CcTPS72 and the linalool synthase gene CcTPS74,suggesting that these transcription factors could be involved in regulating the biosynthesis of the eco-nomically important monoterpenes,borneol and linalool,in Cinnamomum camphora.