水稻PR1基因家族的鉴定与生物信息学分析OA

The pathogenesis-related protein 1(PR1)family plays a key role in plant defense mecha-nisms.To gain a deeper understanding of the functions and characteristics of the PR1 gene family in rice(Oryza sativa L.),bioinformatics methods were employed to comprehensively identify and ana-lyze the members of the PR1 gene family in the rice genome.The results showed that a total of 27 rice PR1 genes were identified,encoding proteins ranging from 51 to 418 amino acids in length,with theo-retical isoelectric points between 4.23 and 10.57 and relative molecular masses ranging from 5 345.94 to 43 345.60 Da.These proteins were primarily localized in the extracellular space and chloroplasts,with random coils dominating their secondary structures,and phosphorylation sites were mainly con-centrated on serine residues.The PR1 genes were distributed across eight chromosomes of rice,with chromosome 7 containing 11 genes.Conserved motifs Motif1 and Motif2 were highly conserved in all members,whereas the numbers of exons and introns varied.Hormone-responsive elements(39.38％)and light-responsive elements(37.62％)were predominant,with the methyl jasmonate(MeJA)-re-sponsive element showing the highest frequency.Phylogenetic analysis classified the PR1 proteins into 8 major groups.The PR1 gene family of rice showed high homology with that of maize,and the evolu-tionary pathway was conserved.The PR1 family genes exhibited marked differentiation across differ-ent tissues.