家畜诱导性多能干细胞培养体系研究进展OA

The establishment of induced pluripotent stem cells(iPSCs)represents a revolutionary breakthrough in stem cell research.After years of development,compared to the mature reprogramming techniques and culture methods for human and murine iPSCs,livestock iPSCs-while capable of sustaining proliferation in vitro and possessing limited differentiation potential-have yet to achieve in vivo pluripotency gold standards such as germline transmission.Furthermore,critical aspects including culture systems,definitive marker characterization,and differentiation efficiency remain significantly underdeveloped.This review outlines the initial materials and methods used in livestock iPSC reprogramming,discusses the applications and efficiency variations of different somatic cell types in generating livestock iPSCs,and evaluates the advantages and limitations of various delivery vectors as well as the roles of reprogramming factors in safety and efficiency.It also systematically examines how culture conditions:including feeder cells,basal media,small molecule compounds,and environmental factors—affect the stability of livestock iPSCs.Finally,the potential applications of livestock iPSCs in breeding,biomedical research,and cell-cultured meat production are explored,providing insights and directions for future advancements in this field.