电针调节胎盘11β-HSD2对孕期尼古丁暴露诱发子代肺发育不良的影响OA

Objective To explore the mechanism of electroacupuncture(EA)protection against pulmonary dysplasia induced by nicotine exposure during pregnancy from the perspective of placental 11β-hydroxysteroid dehydrogenase 2(11β-HSD2)regulating the content of glucocorticoids in offspring.Methods Totally 18 pregnant female SD rats were randomly divided into control group,model group and EA group,with 6 rats in each group.Nicotine(1 mg/kg,100 μL)was injected to induce the pulmonary dysplasia model in offspring.In the EA group,EA was applied to the"Zusanli"acupoint once daily for 20 minutes.On the 20th day of pregnancy,the number of offspring rats,placental mass and body mass were recorded,and the general condition and respiratory score of offspring rats were evaluated,lung mass and lung index were measured,the lung tissue morphology of offspring rats was observed by HE staining,the serum corticosterone content of offspring rats was detected by high performance liquid chromatography triple quadrupole tandem mass spectrometry,the mRNA and protein expressions of placental 11β-HSD2 and glucocorticoid receptor(GR)in lung tissue of offspring rats were detected by RT-PCR and Western blot.Results Compared with the control group,the model group exhibited significant reductions in placental mass,offspring body mass,lung mass,lung index and respiratory scores(P<0.01),a marked decrease in alveolar number along with a significant increase in alveolar area(P<0.01),significantly elevated serum corticosterone(CORT)content(P<0.01),significantly lower placental 11β-HSD2 mRNA and protein expression,and significantly higher pulmonary GR mRNA and protein expression(P<0.01).Compared with the model group,the EA group showed increases in placental mass,offspring body mass,lung mass,lung index and respiratory scores(P<0.01,P<0.05),a significant rise in alveolar number and a significant reduction in alveolar area(P<0.01),significantly decreased serum CORT content(P<0.01),significantly increased placental 11β-HSD2 mRNA and protein expression,and decreased pulmonary GR mRNA and protein expression(P<0.01).Conclusion EA at"Zusanli"acupoint can protect against prenatal nicotine exposure-induced pulmonary dysplasia in offspring.Its mechanism may be related to the regulation of placental 11β-HSD2 and the mitigation of excessive glucocorticoid exposure in offspring.