大戟醇对MH7A细胞凋亡、迁移及侵袭的影响OA

Objective To investigate the effects of euphol on apoptosis,migration and invasion of rheumatoid arthritis(RA)fibroblast-like synoviocytes MH7A cells.Methods MH7A cells were treated with euphol at concentrations of 0(blank control),3.125,6.25,12.5,25,50,100 μg/mL and methotrexate(MTX)at 20 μg/mL for 12,24 and 48 h.Cell viability was detected by CCK-8 method.The cells were divided into the blank control group,MTX group(20.0 μg/mL),and euphol low-,medium-and high-dose groups(25,50,100 μg/mL),and all cells were cultured in complete MH7A culture medium for 24 h.Flow cytometry was used to detect cell apoptosis,wound healing assay was used to detect cell migration ability,Transwell invasion assay was used to detect cell invasion ability,and enzyme-linked immunosorbent assay was used to detect the expression levels of matrix metalloproteinase-3(MMP-3),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-6(IL-6)in cell supernatant.Results Compared with the blank control,cell viability was significantly decreased after treatment with MTX and euphol at 50 and 100 μg/mL for 12,24 and 48 h(P<0.05).Compared with the blank control group,the apoptosis rates in the MTX group and euphol high-dose group were significantly increased,while the cell migration index and the expression level of TNF-α were significantly decreased(P<0.05);the cell invasion indexes in the MTX group and euphol medium-and high-dose groups,and the expression levels of MMP-3,IL-6 and IL-1β were significantly decreased(P<0.05).Conclusion Euphol can exert anti-RA effects by inhibiting MH7A cell viability,promoting cell apoptosis,and attenuating cell migration and invasion abilities.