荷叶粉对兴国灰鹅生长性能、血清抗氧化指标及肠道菌群的影响OA

[Objective]This experiment aimed to investigate the effects of dietary lotus leaf powder(LLP)supplementation on the growth performance,serum antioxidant capacity,intestinal development,and intestinal microbiota of Xingguo Grey geese.[Method]A total of 240 healthy 22-day-old female Xingguo Grey geese with similar body weights(780.37 g±3.72 g)were randomly divided into 4 groups,with 6 replicates per group and 10 geese per replicate.Geese in control group(CON)were fed a basal diet,while geese in the experimental groups(LLP3,LLP6,and LLP9)were fed diets supplemented with 3%,6%,and 9%LLP,respectively.This experiment was conducted in two phases:From 22 to 49 days of age and from 50 to 70 days of age,with a total trial period of 49 days.The weight of the meat geese was measured on the 22,49,and 70 days of age,respectively,with daily feed consumption was recorded to assess growth performance.Upon completion of the experiment,blood samples,segments of the small intestine,jejunal mucosa and cecal contents were collected by replicate to determine serum immune and antioxidant parameters,intestinal weight and length,jejunal mucosa digestive enzyme activity and cecal microbiota composition in the geese.[Result]①Compared with CON group,dietary supplementation with 6%and 9%LLP significantly reduced the average daily gain(ADG)and significantly increased the feed-to-gain ratio(F/G)of Xingguo Grey geese from 22 to 49 days of age(P<0.05).However,from 50 to 70 days of age,the addition of LLP had no significant effect on ADG,average daily feed intake(ADFI)and F/G(P>0.05).During the entire experimental period(from 22 to 70 days of age),diet added with 3%LLP had no significant impact on the growth performance of Xingguo Grey geese(P>0.05).However,dietary supplementation with 6%LLP significantly decreased ADG of Xingguo Grey geese(P<0.05),while supplementation with 9%LLP significantly reduced ADG and ADFI(P<0.05).② Compared with CON group,dietary addition of 3%and 6%LLP significantly increased the serum glutathione peroxidase(GSH-Px)activity of Xingguo Grey geese at 49 days of age(P<0.05),while the supplementation with 9%LLP significantly increased the serum superoxide dismutase(SOD)activity at 49 days of age(P<0.05).Additionally,the addition of 6%and 9%LLP significantly reduced the serum tumor necrosis factor-α(TNF-α)content at 49 days of age(P<0.05),and 3%and 6%LLP supplementation significantly reduced the serum interleukin-6(IL-6)level at 70 days of age(P<0.05).③Compared with CON group,diet added with 9%LLP significantly increased the relative weights of duodenum,jejunum,and ileum of Xingguo Grey geese(P<0.05),but there were no significant changes in the activities of α-amylase,trypsin,maltase,and lactase in the jejunal mucosa of each addition group(P>0.05).④Compared with CON group,the addition of 3%LLP to the diet significantly reduced the relative abundances of Desulfovibrionota,Actinobacteria and Desulfovibrio in the cecal microbiota of Xingguo Grey geese(P<0.05).Meanwhile,dietary supplementation with 3%and 6%LLP significantly increased the relative abundance of Rikenellaceae_RC9_gut_group(P<0.05)and significantly decreased the relative abundance of Faecalibacterium(P<0.05).[Conclusion]Dietary supplementation with 6%and 9%LLP showed inhibitory effects on the growth performance of Xingguo Grey geese,while 3%LLP supplementation had no negative effects on production performance.Moreover,the 3%LLP addition enhanced antioxidant capacity,improved immune function,promoted intestinal development and optimized cecal microbiota composition.Under the conditions of this experiment,it was recommended that the supplementation level be controlled within 3%.