亚洲兰茂牛肝菌菌塘与非菌塘土壤细菌的差异OA

To investigate the differences in bacterial communities between colonized and non-colonized soils of Lanmaoa asiatica,this study collected colonized and non-colonized soil samples from wild L.asiatica from Fanjingshan.Illumina MiSeq high-throughput sequencing technology was employed to compare the bacterial community structure,species com-position,and metabolic functions of the two soil samples.The results showed that there were no significant differences in α-diversity or β-diversity between the colonized and non-colonized soils,indicating highly similar community structures.The dominant phyla in both soils were Acidobacteria and Proteobacteria,but differentiation was observed at the genus lev-el:Bryobacter dominated in colonized soil,while Bradyrhizobium dominated in non-colonized soil.Among the non-domi-nant taxa,HSB_OF53-F07 and the Burkholderia-Caballeronia-Paraburkholderia complex were significantly more abun-dant in colonized soil than in non-colonized soil,although both had relative abundances below 2%.LEfSe analysis identi-fied three biomarkers enriched in colonized soil(HSB_OF53-F07,JG30a-KF-32,andB12_WMSP1)and three in non-colonized soil(Gaiella_sp_EBR4_RS1,Paenibacillus kobensis,and Acidimicrobiia),and the two groups differed in their biomarker taxa.Tax4Fun functional prediction revealed no significant differences in metabolic functions between the two groups,with both dominanted by carbohydrate and amino acid metabolism.In conclusion,although the soil bacterial communities of colonized and non-colonaized soils were largely similar,colonized soil enriched specific low-abundance taxa such as HSB_OF53-F07 and JG30a-KF-32,which may represent potential growth-promoting bacteria.This study systematically characterizes the core bacterial community characteristics of the soil colonized by L.asiatica,and provides theoretical support for the screening of growth-promoting bacteria and the artificial conservation of this fungus.