表达尼帕病毒F和G囊膜糖蛋白的VSV假病毒的构建与优化OA

To enable safe and effective Nipah virus(NiV)studies under lower biosafety conditions,this research aimed to construct a pseudovirus model that carried a reporter gene and mimics the NiV invasion process.This study utilized the established rVSV-ΔG-EGFP pseudovirus system based on vesicular stomati-tis virus(VSV),where the VSV G glycoprotein gene was replaced with enhanced green fluorescent protein(EGFP)in the viral genome.Following exogenous expression of NiV F and G proteins in cells,cells were transfected with the rVSV-ΔG-EGFP backbone to package the recombinant pseudovirus rVSV-ΔG-NiV F/G-EGFP.Transmission electron microscopy and Western-blot analysis confirmed successful packaging of the pseu-dovirus particles and correct expression of the envelope proteins.Neutralization assays demonstrated that this pseudovirus could be effectively inhibited by NiV-specific neutralizing antibodies.The con-structed pseudovirus system provides a safe and effective technical platform for evaluating NiV neutral-izing antibodies,screening therapeutic candidates,and investigating viral entry mechanisms.