Rosa26位点定点敲入hSCARB2基因小鼠的生理生化及心脏功能表型分析OA

Objective Using CRISPR/Cas9 technology-generated Rosa26 locus hSCARB2 gene targeted knock-in mice as the research subjects,this study systematically measured their blood physiological parameters,serum biochemical indices,and electrocardiographic parameters,and analyzed the effects of sex and genotype on these indicators,so as to provide baseline data for the application of this model in enterovirus-related research.Methods Venous blood was collected from 8 weeks old hSCARB2 knock-in(KI)mice(n=10)and wild-type littermates(n=10),with equal numbers of males and females,to measure 18 hematological and 15 serum biochemical parameters.Electrocardiograms were recorded at the same time point.Independent-samples t-tests were used to compare sex and genotype-related differences.Results No significant sex differences were observed in the hematological parameters(P>0.05).Among the 15 biochemical parameters,only serum albumin was slightly higher in male than female hSCARB2 KI mice(P<0.05).Compared with wild-type mice,hSCARB2 KI mice exhibited lower white blood cell and lymphocyte counts(P<0.05),higher hemoglobin concentrations(P<0.01),lower aspartate aminotransferase levels(P<0.01),and higher serum creatinine(P<0.01),total bilirubin,total bile acid,and lactate dehydrogenase-1 levels(all P<0.05).Electrocardiographic parameters and heart rate did not differ significantly between genotypes(P>0.05).Conclusions Targeted KI of hSCARB2 at the Rosa26 locus did not cause significant organ damage in mice,resulting only in modest changes in hematological parameters and biochemical markers related to in lipid metabolism and renal excretion,without affecting baseline cardiac electrophysiological function.These findings indicate that this model has a stable physiological phenotype and may provide a reliable experimental platform for mechanistic studies of enterovirus pathogenesis,vaccine evaluation,and antiviral drug screening.