当归生姜羊肉汤影响脾肾阳虚大鼠外泌体miRNA的分析OA

Objective To investigate whether the improvement of exosomal vesicle miRNA in rats with spleen-kidney Yang deficiency syndrome through treatment with Angelica sinensis ginger mutton soup is related to energy metabolism.Methods A spleen-kidney Yang deficiency syndrome model was established using maternal-fetal separation and senna leaf gavage.Rectal temperature of rats was measured,blood glucose levels were determined using the glucose oxidase method,and serum insulin and other related indicators were detected by enzyme-linked immunosorbent assay(ELISA).Total exosomal RNA was extracted for concentration measurement,integrity assessment,and miRNA sequencing.Differentially expressed miRNAs were identified through bioinformatics analysis,and functional enrichment analysis of their target genes was subsequently performed.Results The Model group exhibited lower body temperature,T3,and insulin sensitivity indexes compared with Normal group(P<0.05,P<0.01).After intervention with A.sinensis ginger mutton soup,these parameters significantly improved(P<0.05).miRNA sequencing revealed six annotated miRNAs with differential expression in exosomes across all groups,among which rno-let-7b-5p had the highest expression level.rno-miR-122-5p was identified as a key differential miRNA,demonstrating validated regulatory relationships with energy metabolism-related target genes,such as Gys1,Slc7a1,Aldoa,and Fasn.Functional enrichment analysis indicated that differential miRNA target genes were significantly enriched in metabolic pathways,including AMPK signaling,insulin signaling,and fatty acid biosynthesis.Conclusions A.sinensis ginger mutton soup improved energy metabolism disorder in rats with spleen-kidney Yang deficiency syndrome by regulating miRNA expression in exosomes and targeting energy metabolism-related genes and signaling pathways.