穴位埋线通过纠正糖脂代谢紊乱和诱导内脏白色脂肪褐变改善大鼠腹型肥胖OA

Objective To investigate the effects of acupoint catgut embedding(ACE)on obesity,glucose-lipid metabolism,and white adipose tissue(WAT)browning in rats with abdominal obesity(AO),and to explore its potential weight-loss mechanisms.Methods Sixty SD rats were randomly assigned to control(n=10)and modeling groups(n=50).AO models were established through high-fat diet feeding.Thirty successfully modeled rats were randomly divided into model group,ACE,and sham acupoint catgut embedding(SACE)groups(n=10 each).The ACE group received treatment under anesthesia at the single acupoints"Zhongwan and Guanyuan",and bilateral acupoints"Tianshu,Daheng,Daimai,Fenglong and Pishu"once weekly for 8 weeks.The procedure was performed without embedding the thread in the SACE group;the control and model groups received anesthesia only.Body mass,abdominal circumference,Lee's index,food intake,inguinal WAT(iWAT)and epididymal WAT(eWAT)mass,serum lipids(total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)),and insulin-related indices(fasting blood glucose(FBG),fasting insulin(FINS),homeostasis model assessment of insulin resistance(HOMA-IR))were measured.Protein and mRNA expression levels of browning-related markers in eWAT,including uncoupling protein 1(UCP1),peroxisome proliferator-activated receptor gamma coactivator-1 α(PGC-1α),peroxisome proliferator-activated receptor γ(PPARγ),and PR domain containing 16(PRDM16),were assessed using Western Blot and qPCR.Results Compared with the control group,the model group showed increased obesity indices,iWAT and eWAT mass,FBG,FINS,HOMA-IR,TC,and LDL-C,decreased HDL-C,and downregulation of browning-related molecules(P＜0.05 or P＜0.01 or P＜0.001).Compared with the model group,the ACE group showed reductions in obesity indices and WAT mass(iWAT and eWAT),improvements in glucose-lipid metabolism,and upregulation of browning molecules(P＜0.05 or P＜0.01 or P＜0.001);no significant changes were observed in the SACE group versus the model group(P＞0.05).Compared with the SACE group,the ACE group exhibited reduced obesity indices and iWAT mass,improved lipid and insulin-related parameters,and increased expression of browning-related molecules(P＜0.05 or P＜0.01 or P＜0.001),while eWAT mass did not differ significantly(P＞0.05).Correlation analysis further demonstrated that browning-related marker expression in eWAT was significantly associated with improvements in glucose-lipid metabolic parameters.Conclusions Acupoint catgut embedding reduces AO in rats,possibly through regulating glucose-lipid metabolism and promoting visceral WAT browning.