鱼类实验动物稀有鮈鲫的寿命与衰老研究OA

Objective To investigate the lifespan characteristics and aging processes of the laboratory fish Gobiocypris rarus(G.rarus).Methods The survival and growth of 1 440 experimental fish from the same birth cohort were monitored,and vertebral morphology,gamete quality,and DNA methylation(DNAm)levels were analyzed in individuals of different ages.Results The maximum observed lifespan of laboratory G.rarus was 94 months,and the average lifespan was 32.05 months.The survivorship curve fell between Types Ⅱ and Ⅲ,with higher annualized mortality rates before 1 month of age and after 25 months of age.The relationship between body mass(M)and body length(L)was described by the equation M=1.182 × 10-5 L3.149,revealing positive allometric growth and aging characteristics such as growth stagnation among older individuals.No age-related trends were observed in sperm quality,whereas egg quality declined significantly in older fish.Older fish also exhibited obvious vertebal curvature,a characteristic sign of aging.A DNAm-based age prediction model for G.rarus was constructed,showing that DNAm age was higher than chronological age before 24 months of age but lower thereafter,indicating distinct aging patterns before and after 24 months of age.Conclusions G.rarus exhibited multiple age-related phenotypic changes,including growth stagnation,vertebal curvature and decreased egg quality,along with age-dependent changes in the aging marker DNAm.These findings further support the application value of G.rarus as a laboratory model for aging research.