实验用大林姬鼠封闭群生物学特性的系统研究OA

Objective To systematically analyze the biological characteristics of Apodemus peninsulae under standardized feeding conditions,including growth and development,reproductive performance,hematological and biochemical parameters,anatomical and histological characteristics,thereby providing baseline data for the standardization of this species as a laboratory animal.Methods Forty male and 40 female Apodemus peninsulae aged 4 or 8 weeks were included.Body mass and size,reproductive performance,14 hematological and 12 serum biochemical parameters,organ mass and coefficients,and skeletal bone counts were measured.Major organs were examined by hematoxylin-eosin(HE)staining,and liver tissue was evaluated using Oil Red O and Masson's trichrome staining.Results The mean neonatal mass was(2.259±0.382)g.Rapid growth occurred from 14 d of age and slowed after 58 d,with significant sexual dimorphism in body mass.The mean litter size increased from(5.20±1.65)in the F1 generation to(6.42±1.28)in the F4 generation.Multiple hematological and biochemical parameters varied by age and sex.Serum levels of alanine aminotransferase,aspartate aminotransferase,and their serum ALT,AST and TG levels were different from those reported in C57BL/6 mice.At 8 weeks of age,mild hepatic lipid deposition and inflammatory infiltration were observed,accompanied by positive Oil Red O staining.Conclusions This study provides a comprehensive characterization of the biological features of Apodemus peninsulae,establishing baseline reference data to support its standardization as a laboratory animal and the development of disease models.The findings also suggest that Apodemus peninsulae may serve as a spontaneous animal model of metabolic dysfunction-associated steatotic liver disease(MASLD).