野生马麝源海氏肠球菌与蒙氏肠球菌的分离及益生特性研究OA

The purpose of this experiment was to isolate and identify probiotics from the feces of wild musk deer in Shiqu County and study their probiotic characteristics.By diluting musk deer feces,coating screening and repeated purification,four probiotics were finally obtained.After Gram staining microscopy and 16S rDNA gene sequencing,the growth curve,acid and bile salt resistance,in vitro bacteriostasis,drug sensitivity,hemolysis,surface hydrophobicity and self-aggregation test were carried out.The results showed that the No.1,No.2 and No.4 strains were Enterococcus hirae,and the No.3 strain was Enterococcus mundtii.The four strains could survive at pH=3,pH=4,pH=5 and 0.1％,0.2％,0.3％bile salt concentration.There was no resistance to ampicillin,penicillin,erythromycin,tetracycline,minocycline,norfloxacin,ciprofloxacin,chloramphenicol,doxycycline,vancomycin and nitrofurantoin.It also had bacteriostatic effect on Salmonella,Staphylococcus aureus and Escherichia coli.It was non-hemolytic and had a certain surface hydrophobicity and self-aggregation rate.The results suggested that strain 4 had outstanding bile salt tolerance(0.3％)(P<0.05),good growth characteristics,strong surface hydrophobicity and self-aggregation ability,certain antibacterial effect,no drug resistance and hemolysis,and more potential for application in the field of microbial preparations.