灌注5-羟基色氨酸和饲喂频率对细毛羊肠道微生物的影响OA

The experiment was conducted to investigate the effects of 5-hydroxytryptophan(5-HTP)infusion and feeding frequency on intestinal microorganisms of fine wool sheep.Twenty-four healthy Erdos fine wool sheep with similar body condition were selected.A 2×2 completely randomized trial design was adopted,with 5-HTP perfusion as the first factor.Control group and 0.178 mg/kg BW 5-HTP perfusion group were set up.The second factor was feeding frequency.There were four experimental groups with 6 replicates in each group,two feeding groups and five feeding groups.The pre-feeding period was 15 days and the normal feeding period was 30 days.The results showed that infusion of 5-HTP could significantly decrease Spirochaetes and significantly increase the relative abundance of Fibrobacteres at rumen level(P<0.05).The relative abundance of Prevotella_1 at rumen level and Ruminococcaceae_NK4A214 at duodenum level were significantly increased by 5-HTP infusion and increasing feeding frequency(P<0.05).Infusion of 5-HTP could significantly increase the relative abundance of Christensenellaceae_R-7_group at rumen level(P<0.05).Injecting 5-HTP could significantly increase the relative abundance of Actinobacteria at duodenal portal level(P<0.05).Injecting 5-HTP and increasing feeding frequency significantly increased the relative abundance of Firmicutes at ileal level(P<0.05).In conclusion,5-HTP infusion can significantly increase the abundance of beneficial bacteria in the intestine of fine wool sheep and improve the balance of intestinal flora.