绵羊肌肉发育关键基因KLF5的CRISPR/Cas9编辑及对产肉性状的调控机制OA
CRISPR/Cas9 editing of KLF5,a key gene in sheep muscle development,and its regulatory mechanisms for meat production traits
KLF5(Krüppel 样因子 5)作为转录因子家族成员,在细胞增殖、分化及组织发育中发挥重要作用.本研究通过 CRISPR/Cas9 技术靶向编辑绵羊 KLF5 基因,探究其对肌肉细胞增殖分化及产肉性状的调控机制.结果表明:KLF5 敲除可显著促进绵羊肌肉细胞的增殖与分化能力,并上调肌肉发育相关基因(如 MYOD、MYOG)的表达.体内试验进一步验证,KLF5编辑绵羊的肌纤维直径和肌肉质量显著增加.本研究揭示了 KLF5 在绵羊肌肉发育中的负调控作用,为产肉性状遗传改良提供了新靶点.
KLF5(Krüppel-like factor 5),as a member of the transcription factor family,plays an important role in cell proliferation,differentiation,and tissue development.This study targeted the editing of the sheep KLF5 gene using CRISPR/Cas9 technology to explore its regulatory mechanism on muscle cell proliferation,differentiation,and meat production traits.Experimental results indicated that KLF5 knockout significantly promote the proliferation and differentiation capacity of sheep muscle cells and upregulate the expression of muscle development-related genes(such as MYOD and MYOG).In vivo experiments further confirmed that the muscle fibre diameter and muscle mass of KLF5-edited sheep were significantly increased.This study reveals the negative regulatory role of KLF5 in sheep muscle development,providing new targets for the genetic improvement of meat production traits.
王丽娟
甘肃省绵羊繁育技术推广站,甘肃 张掖 734031
农业科技
细胞增殖产肉性状肌肉调控
cellsproliferationmeat production traitsmuscleregulation
《中国饲料》 2026 (17)
15-22,8
国家绒毛用羊产业技术体系(CARS-39-34)
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