火针疗法联合异维A酸软胶囊对痤疮模型小鼠炎症及NLRP3/Caspase-1信号通路的影响OA
Effect of Fire Needle Therapy Combined with Isotretinoin Soft Capsules on Inflammation and NLRP3/Caspase-1 Signaling Pathway in Acne Model Mice
目的:探究火针疗法联合异维A酸软胶囊对痤疮模型炎症及NOD样受体蛋白3(Nod-like Receptor Protein 3,NLRP3)/半胱天冬氨酸蛋白酶-1(Caspase-1)信号通路的影响.方法:将60只小鼠随机分成六组,每组10只:对照组、模型组、火针组、异维A酸软胶囊组、联合治疗组和激活剂组.耳廓注射痤疮丙酸杆菌(C.acnes)构建痤疮模型.观察小鼠耳廓表观形态变化及耳廓肿胀率.HE染色观察耳廓组织病理变化.ELISA检测血清炎症因子水平.免疫组化检测MMPs阳性表达水平.蛋白免疫印迹检测NLRP3/Caspase-1信号通路相关蛋白的表达水平.结果:与对照组比较,模型组的耳廓厚度、肿胀率、TNF-α、IL-6、IL-18和IL-1β水平、MMP-1、MMP-3和MMP-9阳性表达、NLRP3和Caspase-1蛋白表达水平显著增加(P＜0.05).与模型组比较,火针组和异维A酸软胶囊组的耳廓厚度、肿胀率、TNF-α、IL-6、IL-18和IL-1β水平、MMP-1、MMP-3和MMP-9阳性表达、NLRP3和Caspase-1蛋白表达水平显著降低(P＜0.05).与火针组和异维A酸软胶囊组比较,联合治疗组的耳廓厚度、肿胀率、TNF-α、IL-6、IL-18和IL-1β水平、MMP-1、MMP-3和MMP-9阳性表达、NLRP3和Caspase-1蛋白表达水平显著降低(P＜0.05).与联合治疗组比较,激活剂组的耳廓厚度、肿胀率、TNF-α、IL-6、IL-18和IL-1β水平、MMP-1、MMP-3和MMP-9阳性表达、NLRP3和Caspase-1蛋白表达水平显著增加(P＜0.05).结论:火针与异维A酸软胶囊联合疗法可显著改善痤疮小鼠炎症损伤,其作用机制可能与调控NLRP3/Caspase-1信号通路、下调炎症因子及MMPs表达水平相关.
Objective To explore the effect of fire needle therapy combined with isotretinoin acid soft capsule on inflammation and NLRP3/Caspase-1 signaling pathway in acne model.Methods 60 mice were randomly divided into 6 groups,10 in each group:control group,model group,fire needle group,isovitamin A acid soft capsule group,combined treatment group and activator group.An acne model was constructed by injecting Propionibacterium acnes(C.acnes)into the auricle.Observe the apparent morphological changes of the mouse auricle and the swelling rate of the auricle.HE staining was used to observe the pathological changes of auricle tissue.The levels of serum inflammatory factors were detected by ELISA.The positive expression level of MMPs was detected by immunohistochemistry.Western blot was used to detect the expression levels of NLRP3/Caspase-1 signaling pathway-related proteins.Results Compared with the control group,the ear thickness,swelling rate,TNF-α,IL-6,IL-18 and IL-1β levels,MMP-1,MMP-3 and MMP-9 positive expression,NLRP3 and Caspase-1 protein expression levels in the model group were significantly increased(P＜0.05).Compared with the model group,the auricle thickness,swelling rate,TNF-α,IL-6,IL-18 and IL-1β levels,MMP-1,MMP-3 and MMP-9 positive expression,NLRP3 and Caspase-1 protein expression levels in the fire needle group and the isotretinoin acid soft capsule group were significantly decreased(P＜0.05).Compared with the fire needle group and isotretinoin acid soft capsule group,the auricle thickness,swelling rate,TNF-α,IL-6,IL-18 and IL-1β levels,MMP-1,MMP-3 and MMP-9 positive expression,NLRP3 and Caspase-1 protein expression levels in the combined treatment group were significantly decreased(P＜0.05).Compared with the combined treatment group,the auricle thickness,swelling rate,TNF-α,IL-6,IL-18 and IL-1β levels,MMP-1,MMP-3 and MMP-9 positive expression,NLRP3 and Caspase-1 protein expression levels in the activator group were significantly increased(P＜0.05).Conclusion The combined therapy of fire needle and isotretinoin soft capsule can significantly alleviate inflammatory lesions in acne model mice,and its mechanism may be related to the regulation of NLRP3/Caspase-1 signaling pathway to affect the levels of inflammatory factors and MMPs.
陶运;孙碧晨;李领娥;胡立忠;陈丽;刘阳;武宁波;柴旭亚
华北石油管理局总医院皮肤一科 河北 沧州 062552河北中医药大学 河北 石家庄 050000石家庄市中医院皮肤科 河北 石家庄 050000华北石油管理局总医院皮肤一科 河北 沧州 062552华北石油管理局总医院皮肤一科 河北 沧州 062552石家庄市中医院皮肤科 河北 石家庄 050000石家庄市中医院皮肤科 河北 石家庄 050000石家庄市中医院皮肤科 河北 石家庄 050000
医药卫生
火针异维A酸软胶囊痤疮NLRP3/Caspase-1炎症MMPs
fire needleisotretinoin acid soft capsuleacneNLRP3/Caspase-1inflammationMMPs
《中国美容医学》 2026 (9)
47-53,7
河北省中医药管理局科研计划项目(编号:2024318)
评论