山东省济宁市部分鹅场舍内空气细菌检测及耐药基因分析OA

Microbial aerosols originating from livestock and poultry farms are major reservoirs for pathogenic microorganisms and antimicrobial resistance genes(ARGs),posing a serious threat to the health of both animals and farm workers.However,limited information has been reported on the composition of aerosol microbial communities within goose barns and their associated ARGs.In this study,airborne bacterial community composition and associated ARG profiles were analyzed using metagenomic sequencing and PCR,targeting two gosling farms(geese aged 10 days)and two growing goose farms(geese aged 50 to 55 days)in Jining,Shandong Province.The results showed that at the phylum level,the airborne bacterial communities were dominated by Proteobacteria and Firmicutes;at the genus level,by Psychrobacter,Corynebacterium,Aerococcus and Staphylococcus;and at the species level,multiple zoonotic opportunistic pathogens were detected,including Aerococcus urinaeequi,Mammaliicoccus sciuri,and Streptococcus alactolyticus.Metagenomic analysis identified a total of 1 424 ARG subtypes,predominantly conferring resistance to fluoroquinolones,tetracyclines,and macrolides.A total of 36 ARG subtypes were tested as positive by a targeted PCR assay on culturable airborne bacteria,covering nine major classes of antibiotics(e.g.,β-lactams and quinolones),and ARG profiles differed among the goose barns.The composition of airborne microbial communities and the characteristics of ARG contamination in goose barns were preliminarily revealed,providing baseline data for evaluating biosafety risks in waterfowl production environments and formulating scientific prevention and control strategies.