大戟脂总三萜对MH7A细胞增殖凋亡迁移和侵袭的影响OA

Objective To investigate the effects of Euphorbium total triterpenoids(TTE)on the proliferation,apoptosis,migration,and invasion of MH7A cells(rheumatoid arthritis fibroblast-like synoviocytes,RA-FLS).Methods The experimental concentrations of TTE were determined as 6.25,12.5,and 25 μg·mL-1 based on CCK-8 results.Furthermore,cell migration and invasion were evaluated using a scratch wound-healing assay and a Transwell invasion assay,respectively,while apoptosis was detected by flow cytometry.The levels of tumor necrosis factor-alpha(TNF-α),interleukin-1beta(IL-1β),and interleukin-6(IL-6)in the cell culture supernatant were measured using enzyme-linked immunosorbent assay(ELISA).Western blotting assay was used to detect the protein expression of PI3K,p-Akt,p-mTOR,MMP-3,MMP-9,and Cleaved Caspase-3 in MH7A cells.Results Compared with the blank control group,TTE significantly inhibited the proliferation,migration,and invasion of MH7A cells and promoted their apoptosis(P<0.05).TTE(12.5 μg·mL-1)could also effectively reduce the level of TNF-α,IL-1β,and IL-6 in the cell supernatant(P<0.05),inhibit the expression of migration-and invasion-related proteins MMP-3 and MMP-9(P<0.05),and promote the expression of the apoptosis-related protein Cleaved Caspase-3(P<0.05).Moreover,TTE(25 μg·mL-1)could down-regulate the expression of PI3K,p-Akt,and p-mTOR proteins in MH7A cells(P<0.05).Conclusions Preventive mechanisms of TTE in RA involve inhibiting the proliferation,migration,and invasion of MH7A cells,as well as promoting their apoptosis.These findings provide a basis for further development of TTE.