烧伤后并发脓毒症患者血清miR-181c、miR-663b表达水平变化及临床价值OA
Changes and clinical value of serum miR-181c and miR-663b levels in patients with sepsis after burns
目的 探究烧伤后并发脓毒症患者血清微小 RNA-181c(miR-181c)和 miR-663b 水平变化及临床价值.方法 选取2019 年1 月—2024 年4 月深圳市龙华区中心医院烧伤整形外科就诊的烧伤后并发脓毒症患者80 例为脓毒症组,另选取烧伤后未并发脓毒症患者 80 例为非脓毒症组.从烧伤患者创面分泌物中培养检测病原菌,采用实时荧光定量 PCR 法检测血清 miR-181c、miR-663b 表达水平;多因素 Logistic 回归分析血清 miR-181c、miR-663b 表达水平对烧伤患者并发脓毒症的影响;受试者工作特征(ROC)曲线分析血清 miR-181c、miR-663b 表达水平对烧伤患者并发脓毒症的诊断价值.结果 烧伤后并发脓毒症患者 80 例标本中,创面分泌物共分离出 94 株病原菌,其中革兰阳性菌 40 株(42.55%)、革兰阴性菌 51 株(54.26%)、真菌 3 株(3.19%);脓毒症组血清 miR-181c、miR-663b 表达水平高于非脓毒症组(t/P=9.476/＜0.001、7.132/＜0.001);多因素 Logistic 回归分析显示,miR-181c 高、miR-663b 高为烧伤患者并发脓毒症的独立危险因素[OR(95%CI)=2.867(1.377～5.967)、3.152(1.422～6.985)];血清 miR-181c、miR-663b表达水平单独及二者联合预测烧伤患者并发脓毒症的曲线下面积(AUC)分别为 0.858、0.811、0.938,二者联合优于各自单独预测价值(Z/P=2.531/0.018、2.486/0.019).结论 烧伤后并发脓毒症患者血清 miR-181c、miR-663b 表达水平升高,两者联合检测对烧伤患者并发脓毒症的诊断价值较好.
Objective To investigate the changes and clinical value of serum microRNA-181c(miR-181c)and miR-663b levels in patients with sepsis after burns.Methods From January 2019 to April 2024,80 burn patients who developed sepsis after admission to the Department of Burn and Plastic Surgery of Longhua District Central Hospital in Shenzhen were selected as the observation group,and another 80 burn patients who did not develop sepsis were selected as the control group.Pathogenic bacteria were cultured and detected from wound secretions of burn patients.Serum levels of miR-181c and miR-663b were measured using quantitative real-time PCR.Logistic regression was used to analyze the influencing factors for sepsis in burn patients,and ROC curves were drawn to evaluate the diagnostic value of serum miR-181c and miR-663b for sepsis in burn patients.Results Among 80 specimens from patients with sepsis following burns,a total of 94 pathogenic strains were isolated,including 40 strains of Gram-positive bacteria(mainly Staphylococcus aureus and Staphylococcus epider-midis)(42.55%),51 strains of Gram-negative bacteria and 3 strains of fungi(3.19%).Serum levels of miR-181c and miR-663b in the observation group were significantly higher than those in the control group(t=9.476,7.132;both P<0.001).High serum miR-181c and high serum miR-663b levels were independent risk factors for sepsis in patients after burns[OR(95%CI)=2.867(1.377-5.967),3.152(1.422-6.985)].The AUC for predicting sepsis in burn patients was 0.858 for serum miR-181c,0.811 for miR-663b,and 0.938 for the combination of both,with the combined prediction being significantly supe-rior to either alone(Z=2.531,2.486;P=0.018,0.019).Conclusion Serum levels of miR-181c and miR-663b are elevated in patients with sepsis after burns.Combined detection of these two biomarkers has good diagnostic value for sepsis after burns.
李维珊;齐德丽;刘坡;刘月明;徐石;林子哲
518110 广东省深圳市龙华区中心医院烧伤整形外科518110 广东省深圳市龙华区中心医院烧伤整形外科518110 广东省深圳市龙华区中心医院烧伤整形外科518110 广东省深圳市龙华区中心医院烧伤整形外科518110 广东省深圳市龙华区中心医院烧伤整形外科518110 广东省深圳市龙华区中心医院烧伤整形外科
医药卫生
烧伤脓毒症微小RNA-181c微小RNA-663b临床价值
BurnsSepsisMicroRNA-181cMicroRNA-663bClinical value
《疑难病杂志》 2026 (9)
1038-1042,5
2025年度广东省中医药局科研项目立项项目(20251329) 2025 Guangdong Provincial Administration of Traditional Chinese Medicine Research Project Approval(20251329)
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